Protein Info for Atu0667 in Agrobacterium fabrum C58

Annotation: glycolate oxidase iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 PF13237: Fer4_10" amino acids 22 to 87 (66 residues), 28.3 bits, see alignment E=5.5e-10 PF13183: Fer4_8" amino acids 22 to 90 (69 residues), 45.4 bits, see alignment E=3.8e-15 PF13534: Fer4_17" amino acids 24 to 91 (68 residues), 39.7 bits, see alignment E=2.4e-13 PF12838: Fer4_7" amino acids 24 to 90 (67 residues), 40.5 bits, see alignment E=1.2e-13 PF12800: Fer4_4" amino acids 24 to 38 (15 residues), 14.4 bits, see alignment (E = 1.6e-05) amino acids 74 to 89 (16 residues), 14.2 bits, see alignment (E = 1.9e-05) PF02754: CCG" amino acids 189 to 276 (88 residues), 53.1 bits, see alignment E=1.2e-17 amino acids 321 to 404 (84 residues), 54.9 bits, see alignment E=3.1e-18

Best Hits

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 100% identity to atu:Atu0667)

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK25 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Atu0667 glycolate oxidase iron-sulfur subunit (Agrobacterium fabrum C58)
MQTSFTSEQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE
NSRPADREVVTHIDRCLSCLACTTTCPSGVDYMHLVDHARAHIEQTYKRPSMDRLIRNLL
VAVLPHPGRFRLALHLARLARPFSGLLAKSPALKPLQSMLALAPAAVPPASASARPGARP
AEGEKRGRVALLTGCAQPVLDPGINEATLRLLARLGIEVVVPRGEGCCGSLVHHMGREEE
ALASARRNVDVWMREMEEGGLDAIIITASGCGTTIKDYGHMLRLDPAYAEKAARVSALAK
DITEYLASLDLPQRQSQGLTVAYHSACSMQHGQKITLAPKQLLKAAGFTVRDPAEGHLCC
GSAGTYNILQPEISSKLKARKVKNIEATRADVIATGNIGCITQIGTGTDMPILHTVELLD
WAYGGPKPARLLV