Protein Info for Atu0656 in Agrobacterium fabrum C58

Annotation: glutamate-cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01436: glutamate--cysteine ligase" amino acids 10 to 457 (448 residues), 608.7 bits, see alignment E=2.4e-187 PF04107: GCS2" amino acids 50 to 354 (305 residues), 266.2 bits, see alignment E=1.9e-83

Best Hits

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to atu:Atu0656)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK33 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Atu0656 glutamate-cysteine ligase (Agrobacterium fabrum C58)
MARDTTDQTPLSSVTELTDYLAAGCRAEADFRIGTEHEKFAFFRKDNSPVPYFGEASISA
LLKGMQEKLGWEPIMDGENIIGLGEQHGMGAISIEPGGQFELSGAPLENLHQTCKESNQH
LATLREVAEPMGIRFLGIGGSPLWTSDETPRMPKSRYAIMTRYMPKVGTKGLDMMYRTCT
IQVNLDFSSEADMRQKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
GVLPFTFSDDFGFKDYVEWALDVPMYFIVRDGKYHDCTHVTFRQFMNGALKGEIAAWEPT
MGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYNQEALDAADALTAD
WSFDEVIALRNAVPAKGLAAEIAGKPLLGIARQVLDISRTGLKNRKRLNGEGQDETVFLS
SLDEVLAKKTTLAEDLLALYNGRWGGSVVPVFEEYQY