Protein Info for Atu0639 in Agrobacterium fabrum C58

Annotation: glycyl-tRNA synthetase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 6 to 297 (292 residues), 451.7 bits, see alignment E=5.2e-140 PF02091: tRNA-synt_2e" amino acids 7 to 297 (291 residues), 464.9 bits, see alignment E=4.4e-144

Best Hits

Swiss-Prot: 100% identical to SYGA_AGRFC: Glycine--tRNA ligase alpha subunit (glyQ) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 100% identity to atu:Atu0639)

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UHP1 at UniProt or InterPro

Protein Sequence (310 amino acids)

>Atu0639 glycyl-tRNA synthetase, alpha subunit (Agrobacterium fabrum C58)
MNPKRSFQALILTLHNYWADKGCAVLQPYDMEVGAGTFHPATTLRALGPKPWKAAYVQPS
RRPSDGRYGENPNRLQHYYQYQVILKPNPSNLQELYLGSLKAIGLDPLLHDVRFVEDDWE
SPTLGAWGLGWECWCDGMEVSQFTYFQQVCGIECSPVAGELTYGLERLAMYVQGVDNVYD
LNFNGREGEEKISYGDVFLQAEQEYSRHNFEFADTSMLHRHFIDAEKECLALLAAGAPGD
NDNQRLHKCVFPAYDQCIKASHIFNLLNARGVISVTERERYIARVRDLAKACGEAFLLTD
AGGLNWNRAA