Protein Info for Atu0609 in Agrobacterium fabrum C58

Annotation: ABC transporter, substrate binding protein (putrescine)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF02030: Lipoprotein_8" amino acids 9 to 108 (100 residues), 20.2 bits, see alignment E=5.9e-08 PF01547: SBP_bac_1" amino acids 36 to 300 (265 residues), 82 bits, see alignment E=2e-26 PF13416: SBP_bac_8" amino acids 43 to 330 (288 residues), 121.5 bits, see alignment E=1.4e-38 PF13343: SBP_bac_6" amino acids 82 to 308 (227 residues), 79.4 bits, see alignment E=7.7e-26

Best Hits

Swiss-Prot: 58% identical to SPUD_PSEAB: Putrescine-binding periplasmic protein SpuD (spuD) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 100% identity to atu:Atu0609)

MetaCyc: 55% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK51 at UniProt or InterPro

Protein Sequence (365 amino acids)

>Atu0609 ABC transporter, substrate binding protein (putrescine) (Agrobacterium fabrum C58)
MKNRSLRLTLALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYDVYD
GNEVLETKLLAGSSGYDVVAPTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERLAKY
DPGNEYAVNYMWGTTGIGYNVAKVKAALGDVPVDSWDVLFKPENAEKLKSCGINILDASD
ETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCISIGWS
GDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMKPEVA
AKASDYVQYANGNLPSQALMDPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNRVWTQ
IKTGT