Protein Info for Atu0589 in Agrobacterium fabrum C58
Annotation: methylenetetrahydrofolate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to FOLD_AGRFC: Bifunctional protein FolD (folD) from Agrobacterium fabrum (strain C58 / ATCC 33970)
KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to atu:Atu0589)MetaCyc: 53% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]
Predicted SEED Role
"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)
MetaCyc Pathways
- folate transformations III (E. coli) (8/9 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
- formaldehyde oxidation VII (THF pathway) (4/4 steps found)
- tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
- folate transformations II (plants) (8/11 steps found)
- formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
- L-histidine degradation III (4/6 steps found)
- folate transformations I (8/13 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (4/9 steps found)
- purine nucleobases degradation II (anaerobic) (14/24 steps found)
- reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
- purine nucleobases degradation I (anaerobic) (5/15 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (18/56 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.5.1.5 or 3.5.4.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8UHU0 at UniProt or InterPro
Protein Sequence (299 amino acids)
>Atu0589 methylenetetrahydrofolate dehydrogenase (Agrobacterium fabrum C58) MAVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQC GFNSIQHTLPEETTQAALLKLVGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVD GLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLSGLNAVVIGRSNLFGKPMGQL LLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAP EKGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF