Protein Info for Atu0530 in Agrobacterium fabrum C58

Annotation: UDP-galactose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 158 (158 residues), 44 bits, E=6.4e-15
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 2 to 326 (325 residues), 409.3 bits, E=5e-127
• PF02719: Polysacc_synt_2
  • amino acids 3 to 175 (173 residues), 50.9 bits, E=5.4e-17
• PF01370: Epimerase
  • amino acids 3 to 253 (251 residues), 195.7 bits, E=3.4e-61
• PF05368: NmrA
  • amino acids 3 to 118 (116 residues), 35.2 bits, E=4.4e-12
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 307 (304 residues), 164 bits, E=2.7e-51
• PF01073: 3Beta_HSD
  • amino acids 4 to 154 (151 residues), 51.7 bits, E=2.8e-17
• PF13460: NAD_binding_10
  • amino acids 7 to 169 (163 residues), 32.7 bits, E=2.8e-11
• PF07993: NAD_binding_4
  • amino acids 65 to 188 (124 residues), 27 bits, E=9.6e-10

Best Hits

Swiss-Prot: 51% identical to EXOB_AZOBR: UDP-glucose 4-epimerase (exoB) from Azospirillum brasilense

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to atu:Atu0530)

MetaCyc: 44% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (2/2 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• stachyose degradation (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• UDP-sugars interconversion (4/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (2/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See A9CK73 at UniProt or InterPro

Protein Sequence (343 amino acids)

>Atu0530 UDP-galactose 4-epimerase (Agrobacterium fabrum C58)
MAVLVTGGAGYIGSHMVWALLDAGEEVVVVDRLSTGSRWAVAPAARFYLGDAADRALLDQ
VFEENHIETIFHFAGSVSVPESISQPLEYYENNTGTTRALVAAAVRSGIRNFIFSSTAAV
YGNQPFDGPVPETAILSPENPYGLSKLASEIMLRDVVQAHDFNYVALRYFNVAGADPQGR
AGPSPTGVANLIKVACEAATGRRDRVEVYGTDYPTADGTGVRDYIHVSDLIDAHMLAMAH
LRAGGGTRTLNCGYGVGYSVLDVLQAVQRESACEFAVIHCPRRPGDIAAMVADSSRIQSE
LGWRPRFNDLATIVRTALQWEAKRQAQQSGRIPPMRRKLAAIG