Protein Info for Atu0517 in Agrobacterium fabrum C58

Annotation: Chemotaxis protein histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 PF01627: Hpt" amino acids 14 to 75 (62 residues), 42.9 bits, see alignment 9.2e-15 PF02895: H-kinase_dim" amino acids 338 to 400 (63 residues), 70.9 bits, see alignment E=2.1e-23 PF02518: HATPase_c" amino acids 448 to 585 (138 residues), 70.1 bits, see alignment E=4.4e-23 PF01584: CheW" amino acids 591 to 721 (131 residues), 90.8 bits, see alignment E=1.2e-29

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to atu:Atu0517)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CK76 at UniProt or InterPro

Protein Sequence (737 amino acids)

>Atu0517 Chemotaxis protein histidine kinase (Agrobacterium fabrum C58)
MESGLLKLNDGDRDPETVNAVFRAVHSIKGGAGAFGLDDLVAFAHVFETTLDCVRSNKLE
PTQDVLKVMLRSADVLADLTNAARDGGSVDESRTRGLVKELEALANGEAVPASAPAPVAV
KAPEPVKAPAAPKPTDESGFEPIPFSFSDFADETTPLMEVPTFQITFKPHASLYSKGNEA
ALLLRDLSRIGDMSVNCDMSDLPALDKLDPEASYLSWTVSIATEKGEEGIRSVFEFAEWD
CDLEITAVVSDASADEEELPMIPVPFDLSALDGDAQDEPAVATPAMEDAYVAATVEAAVA
TTQIAQSVNAAIEKREAAAAPAAAQANANAAANASAGQTIRVDLDRVDRLINLVGELVIN
QAMLSQSVIENDASGTSAVNMGLDELQQLTREIQDSVMAIRAQPVKPVFQRMSRIVREVA
DMIGKQIRLVTEGENTEVDKTVIDKLAEPLTHMIRNAVDHGIETPEKREAAGKNPEGTIK
LSAKHRSGRILIELQDDGAGINRERVRQKAIDNDLIAADANLTDEEIDNLIFAPGFSTAD
KISDISGRGVGMDVVKRSIQALGGRISISSRPGLGSTFTMSLPLTLAVLDGMVVTVAGQT
LVVPLTAIVETLQPEAKNIHSFGSNQRLISIRNSFCPLVDVGRVLNFRPTQADPVEGVAL
LVESEGGGQRALMVDAIQGQRQVVIKSLEANYTHVPGIAAATILGDGRVALILDVDAIVS
ASRGQPLKQEMSLAATG