Protein Info for Atu0423 in Agrobacterium fabrum C58

Annotation: ABC transporter, nucleotide binding/ATPase protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 26 to 271 (246 residues), 408.5 bits, see alignment E=4.6e-127 PF00005: ABC_tran" amino acids 40 to 195 (156 residues), 111 bits, see alignment E=7e-36

Best Hits

Swiss-Prot: 100% identical to PSTB_AGRFC: Phosphate import ATP-binding protein PstB (pstB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 100% identity to atu:Atu0423)

MetaCyc: 57% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UI76 at UniProt or InterPro

Protein Sequence (271 amino acids)

>Atu0423 ABC transporter, nucleotide binding/ATPase protein (Agrobacterium fabrum C58)
MNMLSEAAVEKALDKKMNEVSYKMIGKDVSVYYGEKRALYDVNLNVRENTVTALIGPSGC
GKSTFLRTLNRMNDTIDGCRVTGKITLDTDDIYDQQIDVVELRARVGMVFQKPNPFPKTI
YENIAYGPRIHGLARNKADMDQIVEHSLQKAGLWNEVKDRLLESGTGLSGGQQQRLCIAR
AVAVSPEVILMDEPCSALDPIATAKVEELIHELRTNYTIVIVTHSMQQAARVSQRTAMFH
LGHLVEENETDKMFTNPDDQRTQDYIMGRFG