Protein Info for Atu0347 in Agrobacterium fabrum C58

Annotation: virulence factor MviN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 transmembrane" amino acids 87 to 109 (23 residues), see Phobius details amino acids 129 to 151 (23 residues), see Phobius details amino acids 159 to 181 (23 residues), see Phobius details amino acids 192 to 212 (21 residues), see Phobius details amino acids 233 to 253 (21 residues), see Phobius details amino acids 274 to 295 (22 residues), see Phobius details amino acids 316 to 336 (21 residues), see Phobius details amino acids 352 to 374 (23 residues), see Phobius details amino acids 386 to 406 (21 residues), see Phobius details amino acids 412 to 434 (23 residues), see Phobius details amino acids 450 to 470 (21 residues), see Phobius details amino acids 486 to 511 (26 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 2 to 512 (511 residues), 506.9 bits, see alignment E=3.2e-156 PF03023: MurJ" amino acids 28 to 470 (443 residues), 371.8 bits, see alignment E=4.5e-115 PF13440: Polysacc_synt_3" amino acids 85 to 340 (256 residues), 24.6 bits, see alignment E=1.5e-09

Best Hits

Swiss-Prot: 78% identical to MURJ_RHIME: Probable lipid II flippase MurJ (murJ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to atu:Atu0347)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D1M9 at UniProt or InterPro

Protein Sequence (529 amino acids)

>Atu0347 virulence factor MviN (Agrobacterium fabrum C58)
MSLIGKFATVGTATLGSRIFGFLRETLMAAAVGTGPVADAFNAAFRFPNTFRRLFAEGAF
NSAFVPLFAKEIEANGMEGARRFSEEVFGVLFTVLLALTILMELSMPFIVRTVIAPGFLE
DPVKFDNTVRLATIMFPYLACMSLAAMMGGMLNSLHRYFAAAIAPVFLNIILIGVLALAW
WKNYDPLQVGYALSWGVMAAGLVQLAIVWIAVRNAGMRIGLRRPRLTKNVQRLLVLALPA
AITGGITQINLLINTNIASAGEGVISSLAYADRIYQLPLGVVGIAVATVLLPELARALRG
GHMVEAGSLQNRSVEFVLFLTLPAAAALLVMAEPIVRFLYERGNFSPSATITVAQILGIY
GLGLPAFVLIKAFIPGFFAREDTRTPMIFAAVSVVVNVSLALTLFPRLGGPGIAIAEITA
GWVNAALLFGMLLWRGHWHVDIPLLTRIPRLLLAAALMAGFVHYALTWLSFELSSASSIF
VRAGTIMGLVFTAMLVYFVLAFVSGGADIGMVKRAVRKRGKKTAETEVG