Protein Info for Atu0320 in Agrobacterium fabrum C58

Annotation: ubiquinone/menaquinone biosynthesis methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 PF01209: Ubie_methyltran" amino acids 26 to 257 (232 residues), 291.3 bits, see alignment E=1.4e-90 TIGR01934: ubiquinone/menaquinone biosynthesis methyltransferase" amino acids 30 to 257 (228 residues), 269.8 bits, see alignment E=7.5e-85 PF13489: Methyltransf_23" amino acids 55 to 237 (183 residues), 28.7 bits, see alignment E=3.1e-10 PF13847: Methyltransf_31" amino acids 69 to 192 (124 residues), 64.3 bits, see alignment E=3.3e-21 PF13649: Methyltransf_25" amino acids 74 to 171 (98 residues), 68.6 bits, see alignment E=2e-22 PF08242: Methyltransf_12" amino acids 75 to 173 (99 residues), 42.9 bits, see alignment E=2.1e-14 PF08241: Methyltransf_11" amino acids 75 to 175 (101 residues), 66.3 bits, see alignment E=9.5e-22

Best Hits

Swiss-Prot: 100% identical to UBIE_AGRFC: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03183, ubiquinone/menaquinone biosynthesis methyltransferase [EC: 2.1.1.- 2.1.1.163] (inferred from 100% identity to atu:Atu0320)

MetaCyc: 57% identical to 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (Cereibacter sphaeroides)
RXN-9235 [EC: 2.1.1.201]

Predicted SEED Role

"Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-)" in subsystem Menaquinone Biosynthesis via Futalosine or Menaquinone and Phylloquinone Biosynthesis or Ubiquinone Biosynthesis (EC 2.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.163 or 2.1.1.201

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UIH5 at UniProt or InterPro

Protein Sequence (258 amino acids)

>Atu0320 ubiquinone/menaquinone biosynthesis methyltransferase (Agrobacterium fabrum C58)
MTDARTTAEGGMETSYGFHKVDEGKKQGLVNEVFHKVAKRYDIMNDVMSAGLHRLWKDAM
VSALSPRKDPSYKILDVAGGTGDVAFRIIEASNRLAHATVLDINGSMLAVGEERAAKKKL
SDNLTFVEANAEELPFEANTFDAYTVAFGIRNVPRIDVALKEAYRVLKRGGRLLVLEFSE
VDMPLLDRVYDAWSFNAIPQFGKMITGDAEPYQYLVESIRKFPNQEDFATMIRTAGFSRV
TYTNYTGGIAALHSGWKL