Protein Info for Atu0131 in Agrobacterium fabrum C58

Annotation: purine nucleoside phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 19 to 263 (245 residues), 182.5 bits, E=4.4e-58
• TIGR01697: inosine/guanosine/xanthosine phosphorylase family
  • amino acids 19 to 263 (245 residues), 340.9 bits, E=3.9e-106
• TIGR01698: purine nucleotide phosphorylase
  • amino acids 19 to 263 (245 residues), 364.9 bits, E=1.9e-113

Best Hits

Swiss-Prot: 46% identical to PUNA_BACSU: Purine nucleoside phosphorylase 1 (punA) from Bacillus subtilis (strain 168)

KEGG orthology group: K03783, purine-nucleoside phosphorylase [EC: 2.4.2.1] (inferred from 100% identity to atu:Atu0131)

MetaCyc: 43% identical to xanthosine phosphorylase (Escherichia coli K-12 substr. MG1655)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.- [EC: 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Purine nucleoside phosphorylase (EC 2.4.2.1)" in subsystem Adenosyl nucleosidases or Deoxyribose and Deoxynucleoside Catabolism or Purine conversions (EC 2.4.2.1)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• superpathway of purine deoxyribonucleosides degradation (6/7 steps found)
• ureide biosynthesis (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (5/6 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
• nucleoside and nucleotide degradation (archaea) (3/10 steps found)
• arsenic detoxification (mammals) (7/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.15

Sequence Analysis Tools

See A9CKN6 at UniProt or InterPro

Protein Sequence (266 amino acids)

>Atu0131 purine nucleoside phosphorylase (Agrobacterium fabrum C58)
MTDGLIDILVERLNGLMPRVAIVLGSGLGSLVEEVSDPIRVPYANLPGFPTSGVSGHAGE
LVAGYLGGEPVMMLSGRVHYYEKGDPAAMRAVIEALAGLGVQSLILTNSAGSVRQDMPPG
SVMQITDHINYSGMNPLIGEESDRRFVGMTTAYDPDLMLAMRNAAIRATVPLFGGVYMWF
SGPSFETPAEIRMARVLGADAVGMSTVPEVILARFFGMRVAAASVITNYGAGMTGAELSH
EETKDMAPVGGKRLAAILKEMIAGGA