Protein Info for Atu0110 in Agrobacterium fabrum C58

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 998 transmembrane" amino acids 829 to 841 (13 residues), see Phobius details TIGR00593: DNA polymerase I" amino acids 7 to 998 (992 residues), 952.3 bits, see alignment E=1.7e-290 PF02739: 5_3_exonuc_N" amino acids 7 to 176 (170 residues), 185.4 bits, see alignment E=1.4e-58 PF01367: 5_3_exonuc" amino acids 177 to 270 (94 residues), 110 bits, see alignment E=1.4e-35 PF01612: DNA_pol_A_exo1" amino acids 377 to 582 (206 residues), 100.5 bits, see alignment E=1.9e-32 PF00476: DNA_pol_A" amino acids 615 to 996 (382 residues), 534 bits, see alignment E=4.1e-164

Best Hits

Swiss-Prot: 80% identical to DPO1_RHILE: DNA polymerase I (polA) from Rhizobium leguminosarum

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 100% identity to atu:Atu0110)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CKP5 at UniProt or InterPro

Protein Sequence (998 amino acids)

>Atu0110 DNA polymerase I (Agrobacterium fabrum C58)
MKKGDHLFLVDGSGFIFRAFHAIPPLNRKSDGLPVNAVSGFCNMLWKLLKDARNTDVGVT
PTHFAVIFDYSSKTFRNELYDLYKANRSAPPEDLVPQFGLIREATRAFNLPCIETEGFEA
DDIIATYARQAEAIGADVTIISSDKDLMQLVTANVHMYDAMKDKQIGVPDVIEKWGVGPE
KMIDLQAMTGDSTDNVPGIPGIGPKTAAQLLEEYGDLDTLLARAGEIKQQKRRENIIANA
ELARLSRQLVALRTDVPLEQSLEELVLEPQNGPKLIAFLKAMEFTTLTRRVAEATDSDAS
AIDPANVPVQWGADAHGPDLDAPAAAMAGDVSAEGNNRPASAVAPAKRMIGEGSTPADLT
TARQALFAATKIDTTAYTAIRDIATLDLWIAAAREAGVVAFDTETTSLDPMQAELVGFSL
AIADNGKDASGTDIRAAYVPLTHKTGSGGDLFSDGIKLAEGQAPFAEALERLKDLLEDEA
VLKVAQNLKYDYLLMKRHGVVMRSFDDTMLISYVLEAGKTTHGMDTLSERWLGHTPIAYK
DVTGSGKSSITFDFVNIDKATAYAAEDADVTLRLWMALKPRLVSERLTKVYERLERPLVP
VLAHMEERGITVDRQILSRLSGELAQKAASFEEEIYELAGERFNIGSPKQLGDILFGRMG
LPGGSKTKTGQWSTSAQVLEDLAAEGAELPRKIVDWRQLTKLKSTYTDALPSYIHPETKR
VHTSYALASTTTGRLSSSEPNLQNIPVRTAEGRKIRTAFISTPGHKLLSADYSQIELRVL
AHVADIPQLRNAFENGIDIHAMTASEMFGVPVEGMPSEVRRRAKAINFGIIYGISAFGLA
NQLSIARSEAGDYIKKYFERFPGIRDYMEATKAFARENGYVETIFGRRAHYPEIRSSNPS
VKAFNERAAINAPIQGSAADIIRRAMIRIEPALEAEKLSARMLLQVHDELIFEVEEAEIE
KTLPVVVSVMEDAAMPAISMRVPLKVDARAADNWDEAH