Protein Info for Atu0087 in Agrobacterium fabrum C58

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 PF08364: IF2_assoc" amino acids 15 to 51 (37 residues), 30 bits, see alignment (E = 2.4e-10) TIGR00487: translation initiation factor IF-2" amino acids 330 to 913 (584 residues), 796 bits, see alignment E=2.4e-243 PF04760: IF2_N" amino acids 335 to 386 (52 residues), 41.3 bits, see alignment 5.2e-14 TIGR00231: small GTP-binding protein domain" amino acids 413 to 568 (156 residues), 108.1 bits, see alignment E=3.8e-35 PF00009: GTP_EFTU" amino acids 416 to 571 (156 residues), 121.9 bits, see alignment E=1.3e-38 PF02421: FeoB_N" amino acids 416 to 570 (155 residues), 31 bits, see alignment E=8.6e-11 PF01926: MMR_HSR1" amino acids 416 to 521 (106 residues), 43.5 bits, see alignment E=1.6e-14 PF00071: Ras" amino acids 417 to 572 (156 residues), 26.3 bits, see alignment E=2.5e-09 PF22042: EF-G_D2" amino acids 587 to 665 (79 residues), 103 bits, see alignment E=3.9e-33 PF11987: IF-2" amino acids 691 to 803 (113 residues), 146.6 bits, see alignment E=1.5e-46 PF03144: GTP_EFTU_D2" amino acids 834 to 901 (68 residues), 39.5 bits, see alignment 3.1e-13

Best Hits

Swiss-Prot: 100% identical to IF2_AGRFC: Translation initiation factor IF-2 (infB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 100% identity to atu:Atu0087)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UJ51 at UniProt or InterPro

Protein Sequence (913 amino acids)

>Atu0087 translation initiation factor IF-2 (Agrobacterium fabrum C58)
MTDNNDDKTLNAPAKKTLTLKPGGMNQGTVRQDMGRGRTNAVVVETRKRRPHRPEDEKPV
QPVVAAPKPAAPAPVAARPQAPQPRIHQPGGQQQRPGSSQSQQRSGSSAPQQRQADRPRG
NVLHDLSAGEMEARRRALMEAQARDVVEAKQRAEDEARRKVEEEQRIAAEKMEAANRAAE
EAAAAKVAASQPAAEVRAEPASERPAAAAAPAPRTDARPQSAAAAPRSAPATPDAAAPRG
RRTGGDDEDDRGAVRRGSSLPARGKVVAPAPAKPAARLKTEEERRRGKLTVTSNLEEDGT
PRGRSMASMRRRQEKFRRSQMQETREKVLREVILPETITIQELSQRMSERAVDVIKFLMK
EGQMLKPGDVIDADLAELIAVEFGHTVKRVSESDVEEGIFNQTDDEGEMVSRPPVVTIMG
HVDHGKTSLLDAIRQANVVSGEAGGITQHIGAYQVEKNGHKITFIDTPGHAAFTAMRARG
AQATDIAVLVVAADDSVMPQTIESINHAKAAGVPIVVAINKIDKHEANPDKVRQQLLQHE
VFVESMGGEVLDVEVSAKNKLNLDKLLEAILLQAEILDLKADPSRTAEGTVIEAELDRGR
GAVATVLVQKGTLKPGQIIVAGDQWGRVRALVNDKGDHVKEAGPAMPVEILGLSGTPSAG
DRFAVVENESRAREISEYRQRLARDKAVARQTGQRGSLEQMMSQLQTSGLKEFPLVIKAD
VQGSVEAIIASLDKLGTDEVRARVVHSGAGAITESDISLAEASNAAIIGFNVRANAQART
ASERAGIEIRYYNIIYDLVDDVKAAMSGLLSPERRETFLGNAEILEVFNITKVGKVAGCR
VVEGKVERGAGVRLVRDNVVIHEGKLKTLKRFKDEVNEVPVGQECGMAFENYEDIRAGDT
IECFRVEHITRTL