Protein Info for Atu0082 in Agrobacterium fabrum C58

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 713 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 10 to 692 (683 residues), 1073.1 bits, see alignment E=0 PF01138: RNase_PH" amino acids 13 to 144 (132 residues), 115.9 bits, see alignment E=4.4e-37 amino acids 325 to 457 (133 residues), 100.4 bits, see alignment E=2.7e-32 PF03725: RNase_PH_C" amino acids 147 to 211 (65 residues), 51.8 bits, see alignment E=1.6e-17 amino acids 461 to 528 (68 residues), 26.9 bits, see alignment E=9.5e-10 PF03726: PNPase" amino acids 241 to 321 (81 residues), 75.5 bits, see alignment E=9.3e-25 PF00013: KH_1" amino acids 557 to 614 (58 residues), 43.6 bits, see alignment 5.1e-15 PF00575: S1" amino acids 619 to 691 (73 residues), 60.1 bits, see alignment E=5.5e-20

Best Hits

Swiss-Prot: 100% identical to PNP_AGRFC: Polyribonucleotide nucleotidyltransferase (pnp) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to atu:Atu0082)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CKQ7 at UniProt or InterPro

Protein Sequence (713 amino acids)

>Atu0082 polyribonucleotide nucleotidyltransferase (Agrobacterium fabrum C58)
MFNKHSVEIEWAGRPLKLETGKVARQADGAVIATYGETMVLATVVSAKSPKPGQDFFPLT
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVIQH
DLENDPDVLSMVAASAALTLSGIPFMGPVGGARVGYINGEYVLNPHLDEMDESVLDLVVA
GTQDAVLMVESEAKELNEEIMLGAVMFGHKGFQPVIDAIIKLAEVAAKEPREFEPEDFSA
LENEMLGLAETELRTAYKITEKAARYAAVDAVKTKVKAHFLPEEGEAKYSPEEIGAVFKH
LQAKIVRWNVLDTKSRIDGRDLSTVRPIVSEVGILPRTHGSALFTRGETQAIVVATLGTG
EDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPTAE
QFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKIALEQAQGGRKHILGEMAN
AITESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIAS
ASGKEIEAARKWIHSIVAEPEVGQIYEGTVVKTADFGAFVNFFGARDGLVHISQLASERV
AKTSDVVKEGDKVWVKLMGFDERGKVRLSMKVVDQATGKEVAADKKDGEAAAE