Protein Info for Atu0047 in Agrobacterium fabrum C58

Annotation: malate synthase G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 27 to 741 (715 residues), 1194.3 bits, E=0
• PF20656: MS_N
  • amino acids 37 to 95 (59 residues), 48.1 bits, 1.3e-16
• PF20658: MSG_insertion
  • amino acids 180 to 252 (73 residues), 90.6 bits, E=1.2e-29
• PF01274: MS_TIM-barrel
  • amino acids 356 to 591 (236 residues), 220.6 bits, E=3.5e-69
• PF20659: MS_C
  • amino acids 610 to 699 (90 residues), 80.2 bits, E=3.5e-26

Best Hits

Swiss-Prot: 100% identical to MASZ_AGRFC: Malate synthase G (glcB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 100% identity to atu:Atu0047)

MetaCyc: 64% identical to malate synthase G (Mycobacterium tuberculosis H37Rv)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• glyoxylate cycle (6/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (3/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• D-xylose degradation IV (3/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)
• superpathway of pentose and pentitol degradation (16/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See Q8UJ85 at UniProt or InterPro

Protein Sequence (744 amino acids)

>Atu0047 malate synthase G (Agrobacterium fabrum C58)
MPRLTSDRLSVQSMPSEYKEAHVSRTDKFGLSIDDRLYAFLTDEVLPGTGLDSETFFEGF
SAIVHELSPKNRELLAKRDALQEKIDGWYRENGAPSDFDAYEAFLKEIGYLLPEGPGFKV
ETNNVDPEIAVVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAISDADGAEKGRGY
NPKRGDKVIAWARNFLDESAPLETGSWSDVTGFNIADGLLQLAIGAATTGLKDAVQFKGF
SGEAAKPATILLGKNGLHTEIVIDPSTEIGKSDRAGISDVILESALTTIMDCEDSVAAVD
AEDKVLVYGNWLGLMRGDLTEAVSKGGNTFTRRLNPDRYYTAPDGSALTLPGRSLMLVRN
VGHLMTNPAILDRDGRDVPEGIMDAVVTALIALYDVGPSGRRQNSRAGSMYVVKPKMHGP
EEVAFANEIFARVENLVGMAPNTMKMGIMDEERRTTVNLKESIRAAKDRVVFINTGFLDR
TGDEIHTSMEAGPMIRKGDMKQAAWIAAYENWNVDIGLECGLSGHAQIGKGMWAMPDLMA
AMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVAAVQEGLKSRGRAKLSDILSVPV
APRPNWTPEEIQRELDNNAQGILGYVVRWVDQGVGCSKVPDINNIGLMEDRATLRISAQH
MANWLRHGVVTEAQIIKTMKRMAAVVDTQNAGDPAYLPMASDFDGSVAFQAAVELVLKGR
EQPNGYTEPVLHRRRLELKAKQAG