Protein Info for Atu0012 in Agrobacterium fabrum C58

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 811 TIGR01059: DNA gyrase, B subunit" amino acids 15 to 811 (797 residues), 990.1 bits, see alignment E=2.4e-302 PF02518: HATPase_c" amino acids 42 to 146 (105 residues), 60.9 bits, see alignment E=3.8e-20 PF00204: DNA_gyraseB" amino acids 233 to 402 (170 residues), 186.5 bits, see alignment E=7.8e-59 PF01751: Toprim" amino acids 431 to 543 (113 residues), 67.2 bits, see alignment E=3e-22 PF21249: GyrB_hook" amino acids 545 to 571 (27 residues), 32.2 bits, see alignment (E = 1.6e-11) PF00986: DNA_gyraseB_C" amino acids 739 to 800 (62 residues), 95.1 bits, see alignment 5.6e-31

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to atu:Atu0012)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CKT5 at UniProt or InterPro

Protein Sequence (811 amino acids)

>Atu0012 DNA gyrase subunit B (Agrobacterium fabrum C58)
MTETSSSEVGANAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDE
ALAGHADLVTVTLNADGSVTVTDNGRGIPTDIHSSEGVSAAEVIMTQLHAGGKFDQNSYK
VSGGLHGVGVSVVNALSVWLKLRIRRNGKLHEIGFTHGVADAPLSVIGEYEGRSGTEVTF
LASPQTFTMTDYDYGTLEHRLRELAFLNSGVRILLTDKRHSDVKQQELLYDGGLEAFVRY
LDRAKKPLVDKPIAIHSEKDGITVEVALWWNDSYHENVLCFTNNIPQRDGGTHMAGFRAA
LTRQVTSYADTSGIMKREKVSLQGEDCREGLTAILSVKVPDPKFSSQTKDKLVSSEVRPV
VESLVNEALSTWLEEHPSEAKILIGKVVEAAVAREAARKARELTRRKGALDIASLPGKLA
DCSERDPAKSELFLVEGDSAGGSAKQGRSRETQAILPLRGKILNVERARFDKMLSSQEIG
TLITALGTSIGKDEFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHLY
IAQPPLYKVTRGKSVQYLKDEKALEEYLISMGIEEASLTLGTGEVRVGADLREVILDALR
MRSLIDGLHSRYSRSIVEQAAIAGALNPELSADAARAQETVAEVARRLDMIAEETERGWS
GVVLEDGGLRFERMVRGVKEISTLDMGLLGSADARHIDQLVARTRDIYATPPVLQRKDGS
MELAGPRALLDAIFAAGRKGLSMQRYKGLGEMNAEQLWETTLDANVRSLLQVRVNDATDA
DGLFARLMGDEVEPRRDFIQENALNVANLDI