Protein Info for AMB_RS22465 in Magnetospirillum magneticum AMB-1

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 TIGR02350: chaperone protein DnaK" amino acids 3 to 599 (597 residues), 997.6 bits, see alignment E=9.1e-305 PF00012: HSP70" amino acids 4 to 599 (596 residues), 930.2 bits, see alignment E=4.4e-284 PF06723: MreB_Mbl" amino acids 130 to 372 (243 residues), 58.6 bits, see alignment E=5.1e-20

Best Hits

Swiss-Prot: 100% identical to DNAK_MAGSA: Chaperone protein DnaK (dnaK) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to mag:amb4440)

MetaCyc: 68% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2VYT1 at UniProt or InterPro

Protein Sequence (642 amino acids)

>AMB_RS22465 molecular chaperone DnaK (Magnetospirillum magneticum AMB-1)
MSKVIGIDLGTTNSCVAVMEGKNAKVIENAEGMRTTPSMTAFTESGERLVGQPAKRQAVT
NPTSTLFAIKRLIGRRFEDPITKKDMNLVPYHIVAGDNGDAWVEARDAKYSPSQVSAFIL
QKMKETAEGYLGEKVTQAVITVPAYFNDAQRQATKDAGRIAGLEVLRIINEPTAAALAYG
LEKKGAGTIAVYDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDARIMDYLADEFK
KEQGIDLRKDRLALQRLKEAAEKAKIELSSSMQTEVNLPFITADASGPKHLNIKLTRSKL
EALVEDLVARTVEPCKAALKDAGVKASEIDEVILVGGMTRMPKIQEVVKEFFGREPHKGV
NPDEVVAIGAAIQGGVLKGEVKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTRKSQV
FSTAEDNQTAVTIRVFQGEREMAADNKVLGQFDLVGIPPAPRGVPQVEVTFDIDANGLVN
VSAKDKATGKEQQIRIQASGGLSDADIEKMVKEAEAHAAEDKKRKELIEARNHADGLIHT
TEKSLKEFGDKAGAELTGAIEKEITALKAVMDGDDVEAIKAKTESLMQASMKLGEAMYKA
QEAAGGAEAEAAAGGHGGASGSHDDKVVDADFEEVDGDKKGK