Protein Info for AMB_RS22355 in Magnetospirillum magneticum AMB-1

Annotation: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 3 to 319 (317 residues), 414.1 bits, see alignment E=3.5e-128 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 4 to 312 (309 residues), 316.7 bits, see alignment E=1.6e-98 PF00814: TsaD" amino acids 23 to 313 (291 residues), 300.7 bits, see alignment E=6e-94

Best Hits

Swiss-Prot: 100% identical to TSAD_MAGSA: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 100% identity to mag:amb4419)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2VYV2 at UniProt or InterPro

Protein Sequence (347 amino acids)

>AMB_RS22355 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD (Magnetospirillum magneticum AMB-1)
MLVLGIESSCDETAAAVVNDRREILGEVVLSQLDEHRPFGGVVPEIAARSHLAHMDKLVA
EAMRRAGVGFADLDAVAATGGPGLIGGVIVGVMTGKAIALAAGKPFLAVNHLEGHALTPR
LTHDIAFPYLLLLASGGHCQLLAVEGVGRYTRLGTTIDDAAGEAFDKVAKMAGLGYPGGP
AVEAAAQGGDPARFTLPRPMKGKPGCDFSFSGLKNAARLLIESLPQPLSAADQADVARAF
QDAVADAMADRVRRGVREMKSRWPQTQHLVVAGGVAANTALRQVLVRIGNETGLEFLAPP
LKLCTDNAAMIAWAGLERLRLGQSDDLTFAPRPRWPLDPTARKGAKA