Protein Info for AMB_RS22220 in Magnetospirillum magneticum AMB-1

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 PF05524: PEP-utilisers_N" amino acids 25 to 150 (126 residues), 98.9 bits, see alignment E=3.5e-32 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 25 to 590 (566 residues), 537.4 bits, see alignment E=1.9e-165 PF00391: PEP-utilizers" amino acids 177 to 249 (73 residues), 69.3 bits, see alignment E=2.6e-23 PF02896: PEP-utilizers_C" amino acids 278 to 566 (289 residues), 338.7 bits, see alignment E=4.3e-105

Best Hits

Swiss-Prot: 38% identical to PT1_SALTY: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 100% identity to mag:amb4393)

MetaCyc: 38% identical to PTS enzyme I (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate--protein phosphotransferase. [EC: 2.7.3.9]

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2VYX8 at UniProt or InterPro

Protein Sequence (596 amino acids)

>AMB_RS22220 phosphoenolpyruvate--protein phosphotransferase (Magnetospirillum magneticum AMB-1)
MTANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAE
CARFNEASANAGRQVEQLQDKAGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRND
RINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRRLLRALTNTAYRPFTALPRNA
VIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGE
QVIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANME
LPNEVGAVLDSGAEGIGLLRSEFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDA
GGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAAILRASAHGPVRILIPMIATV
GELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTND
LVQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLL
LGLGLRDLSMSTSNLPVVKNRIRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH