Protein Info for AMB_RS20115 in Magnetospirillum magneticum AMB-1

Annotation: prephenate dehydrogenase/arogenate dehydrogenase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details signal peptide" amino acids 25 to 25 (1 residues), see Phobius details PF03807: F420_oxidored" amino acids 8 to 99 (92 residues), 28.9 bits, see alignment E=2.8e-10 PF03446: NAD_binding_2" amino acids 9 to 174 (166 residues), 25.9 bits, see alignment E=1.8e-09 PF02153: PDH_N" amino acids 21 to 175 (155 residues), 131.8 bits, see alignment E=3.1e-42 PF20463: PDH_C" amino acids 178 to 279 (102 residues), 101.8 bits, see alignment E=4.8e-33

Best Hits

Swiss-Prot: 41% identical to TYRC_ZYMMO: Cyclohexadienyl dehydrogenase (tyrC) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K00220, cyclohexadieny/prephenate dehydrogenase [EC: 1.3.1.12 1.3.1.43] (inferred from 100% identity to mag:amb3975)

Predicted SEED Role

"Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W046 at UniProt or InterPro

Protein Sequence (294 amino acids)

>AMB_RS20115 prephenate dehydrogenase/arogenate dehydrogenase family protein (Magnetospirillum magneticum AMB-1)
MTQPLFDTVCFVGIGLIGSSLARAMRKHGLARRILTLDPSEKARQTALELGVVDAASDNP
AALIPESDLVVIGAPVGATQAVGAAIGPHLVPGTIVTDVGSVKLSVIRDLGPHIPDGVEL
VPGHPIAGTEHSGPENGFAELFEGRWHILTPVTGGDPKAVDKVAELWRKVGSQVEIMDPH
HHDKVLAITSHLPHLIAYTIVGTANDLEGHMQQEVIKFSASGFRDFTRIAASDPVMWRDV
FLNNREAVLEVIQRFTEDLTALQRAIRWGEGEVLHKRFSETRAIRRAIIDAKQA