Protein Info for AMB_RS19820 in Magnetospirillum magneticum AMB-1

Annotation: cobaltochelatase subunit CobT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 PF06213: CobT" amino acids 18 to 281 (264 residues), 265.2 bits, see alignment E=7.8e-83 TIGR01651: cobaltochelatase, CobT subunit" amino acids 18 to 618 (601 residues), 787.7 bits, see alignment E=4.3e-241 PF11775: CobT_C" amino acids 399 to 615 (217 residues), 336.3 bits, see alignment E=8.8e-105

Best Hits

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 100% identity to mag:amb3916)

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W0A5 at UniProt or InterPro

Protein Sequence (629 amino acids)

>AMB_RS19820 cobaltochelatase subunit CobT (Magnetospirillum magneticum AMB-1)
MSQNATAKDSGRGESIGETIRRATAATLKAMSGHADLDVGYAAGNGSVRGTEIRLPAPPR
NILPSDLARLRGTADAVALRLRYHDDTVHVRRMPQLPEARKIYDMVEQARVEALGARLMP
GAAANIAEVLEVKARAEGLDRAVKREQVALADAMRFIAREIFAGLEPPPSARPAVDLWRD
EVRAKAGPTLDALKDLLRDQEAFAKGLHRLLSDLDFGTDQASEAEDSENSEQSGQEQDAQ
GAGQEKEGEQEGQGPQQEGDTQAPGSSEGSGEDDGTDSGEEMMLTGSGTEEPGGPSKQAQ
EQLSNSAAFQDRPYKPYTTQYDQIVTAEDLCEPEELNRLRRQLDQQLTHLQGVVAKLANR
LQRKLMAQQTRNWDFDLEEGMLDTGRLARIVANPMHSLSFKIEKETNFRDTVVSLLIDNS
GSMRGRPITVAAMSADLLARTLERCGVKVEVLGFTTRAWKGGSAREKWQAEGKPTNPGRL
NDLRHIVYKAADAPWRRVRRNLGLMLREGILKENIDGEALMWAHDRLISRSEQRRILMVI
SDGAPVDDSTLSANPGNTLEKHLRDVIAFIENRSPVELVAIGIGHDVTRYYRRAVTIMDA
EDLGGTMMRQLTALFDDEAEPAGGRRRAR