Protein Info for AMB_RS17865 in Magnetospirillum magneticum AMB-1

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 6 to 560 (555 residues), 715.6 bits, E=6.1e-219
• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 859 (854 residues), 954.6 bits, E=2.7e-291
• PF07973: tRNA_SAD
  • amino acids 659 to 702 (44 residues), 61.6 bits, 8.2e-21
• PF02272: DHHA1
  • amino acids 776 to 876 (101 residues), 55.1 bits, E=1.4e-18

Best Hits

Swiss-Prot: 100% identical to SYA_MAGSA: Alanine--tRNA ligase (alaS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 67% identity to azl:AZL_a04560)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (18/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• superpathway of chorismate metabolism (38/59 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See Q2W1D9 at UniProt or InterPro

Protein Sequence (878 amino acids)

>AMB_RS17865 alanine--tRNA ligase (Magnetospirillum magneticum AMB-1)
MQTANEIRRTFLDFFEKNGHTVVNSSPLVPRNDPTLMFTNAGMVQFKNVFTGIEKRDYVR
AASSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKDLAIEHAWNLITKDFGI
DKSRLLVTVYHDDDQAADAWKKIAGLPESRIIRIPTSDNFWAMGDTGPCGPCSEIFYDHG
EHIFGGPPGSPDQDGDRFIEIWNLVFMQFEQVDKETRIPLPKPSIDTGMGLERLAALLQG
KHDNYDVDLLRALIEASAEASNTDPDGPHKVSHRVVADHLRSSSFLIADGVLPSNEGRGY
VLRRIMRRAMRHAHLMGCTEPLLHKLVPALTRQMGEAYPELVRANALITETLKLEETRFK
VTLDKGLKLLDDEITRIGSGQKLAGEVAFKLYDTYGFPLDLTQDALKAKGIGVDTDGFAS
AMERQRAEARKAWAGSGDTATETVWFELREKLGASEFLGYDAEQAEGKIVALVENGKVVE
AVHPGHQVAVIANQTPFYGESGGQMGDTGVITLGTPGTGGKATIAVTDTQKKLGDLIVHF
GTVEGGPVAVGEDAHFAVESTRRDATRGHHSATHLLHAALRDILGDHVTQKGSQVGPDRL
RFDFAHTKALSAEETRAVEAEVNRRIRLNAEVATKVMTPDDAIKAGAMALFGEKYGEEVR
VVSMGDLSTELCGGTHANRTGDIGGFKIVAESAVASGVRRIEAVTGPAFLAWAQAQEDLL
AKAADTLKAAPADLPARIAGLMDDRRKLERELAEVRKQLATGGGQGAATKTVNGITYAGK
VLSGVPAKDLKGMADEIKKQIGSGIIALVSDADGKASIVVCVTEDLTSKHSAVDLVRIGS
EALGGKGGGGRPDMAQAGGPDASAAQAAVDRIEQALGG