Protein Info for AMB_RS17180 in Magnetospirillum magneticum AMB-1

Annotation: Ni/Fe hydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00374: NiFeSe_Hases
  • amino acids 54 to 131 (78 residues), 58 bits, E=4.7e-20
  • amino acids 274 to 464 (191 residues), 54.9 bits, E=4e-19

Best Hits

Swiss-Prot: 50% identical to HOXH_CUPNH: NAD-reducing hydrogenase HoxS subunit beta (hoxH) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00436, hydrogen dehydrogenase [EC: 1.12.1.2] (inferred from 100% identity to mag:amb3396)

MetaCyc: 50% identical to beta subunit (Cupriavidus necator)
Hydrogen dehydrogenase. [EC: 1.12.1.2]

Predicted SEED Role

"NAD-reducing hydrogenase subunit HoxH (EC 1.12.1.2)" in subsystem Hydrogenases (EC 1.12.1.2)

MetaCyc Pathways

• superpathway of hydrogen production (2/2 steps found)
• hydrogen oxidation II (aerobic, NAD) (1/1 steps found)
• hydrogen production II (1/1 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.12.1.2

Use Curated BLAST to search for 1.12.1.2

Sequence Analysis Tools

See Q2W1S5 at UniProt or InterPro

Protein Sequence (496 amino acids)

>AMB_RS17180 Ni/Fe hydrogenase subunit alpha (Magnetospirillum magneticum AMB-1)
MRHQFELETAKTPEALKRIVIEPVSRVEGHGKVTILLDDNNQVHQVRLHIVEFRGFEKFI
EGRPYWEVPVLVQRLCGICPVSHHLAASKAMDMIVNGGILTPTAEKIRRLMHYGQMLQSH
ALHFFHLSSPDLLFGFDSDVAKRNIVGVAAANPDVAKMGVLLRKFGQEVIRVTSGKRVHG
TGSVPGGVNKSLTAEERDYLLKDSDQMIAWSRDAVRIIKGLHRSNPALYDAFGAVPSTMM
SLVDDKGALDFYHGALRARDKDGEVIFDQVSYQGYNTLLTEGVKSWSYMKFPYITTLGEE
KGWYKVGPLARIQNCDSIPTPQAEEERVEFVAHGGAKPMHGSLGFHWARMIEMLCAAETI
KDLLLDPDIMGADLMAEGPRRGEGIGVIEAPRGTLFHHYRVDDNDLVTYANLIVSTTNNN
TAMNVAVRDVAKNYLQGHEITEGLLNHIEVAIRCFDPCLSCATHALGKMPLEVTLVDATG
AEIDRMTKDSEGRIGK