Protein Info for AMB_RS13855 in Magnetospirillum magneticum AMB-1

Annotation: phenylacetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 70% identical to PAAK_PSEPU: Phenylacetate-coenzyme A ligase (paaK) from Pseudomonas putida

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to mag:amb2755)

MetaCyc: 74% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

phenylacetate degradation II (anaerobic) (3/4 steps found)
phenylacetate degradation I (aerobic) (6/9 steps found)
penicillin G and penicillin V biosynthesis (1/4 steps found)
superpathway of phenylethylamine degradation (6/11 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (11/27 steps found)

KEGG Metabolic Maps

Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3L6 at UniProt or InterPro

Protein Sequence (442 amino acids)

>AMB_RS13855 phenylacetate--CoA ligase (Magnetospirillum magneticum AMB-1)
MSKTAHRRVPPAKDLLDPIEIASRDQITALQTERMKWSLTHAYNNVEHYRNKCREKGVHP
DDFKELKDLAKFPFTTKSDLRDTYPFGMFAVPMDEVVRVHASSGTTGKPTVVGYTQNDID
MWANVMARSIRAAGGRRSDKCHVSYGYGLFTGGLGAHYGAERLGCTVIPMSGGQTEKQIQ
LIMDFKPEIIMVTPSYMLAIADEMDRQGIDTKTCPLQVGIFGAEPWTGAMREEIEVRMGI
EAVDIYGLSEVIGPGVACECVETKDGLHLWEDHFYPEIIDPHTGEVLPDGSMGELVFTSL
TKEALPIIRYRTRDLTQLLPGTARSMRRMGKITGRSDDMLIIRGVNVFPTQIEELILKDY
RLAPHYQLEVTRDGHLDSVAVNVEVRADQPYDDSALEAASKDLQHHIKSYIGISTKVNVL
APNSIERSMGKARRVIDKRPKA