Protein Info for AMB_RS13530 in Magnetospirillum magneticum AMB-1

Annotation: periplasmic nitrate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 827 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 29 (27 residues), 19.5 bits, see alignment (E = 9.6e-08) TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 826 (824 residues), 1552.9 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 37 to 89 (53 residues), 71.3 bits, see alignment 8e-24 PF00384: Molybdopterin" amino acids 93 to 567 (475 residues), 247.7 bits, see alignment E=2.8e-77 PF01568: Molydop_binding" amino acids 713 to 821 (109 residues), 94.1 bits, see alignment E=8.1e-31

Best Hits

Swiss-Prot: 76% identical to NAPA_DECAR: Periplasmic nitrate reductase (napA) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 76% identity to aha:AHA_1586)

MetaCyc: 68% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3T1 at UniProt or InterPro

Protein Sequence (827 amino acids)

>AMB_RS13530 periplasmic nitrate reductase subunit alpha (Magnetospirillum magneticum AMB-1)
MSLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGR
VVATQGDPDSPVNRGLNCIKGYFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQA
FDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKLFKAGFRSNNLDPNARHCMAS
AVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVL
STFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYG
LRPTHALEKDQANAATPDKSDPMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYAD
PKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPGNSPFSLTGQPSACGTAREVG
TFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCT
NNMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERR
TQFWRQQVKAPGEAKSDLWQIMEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQV
DKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHAHDLAPFEAYHKARGLRWPVV
DGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRV
LEHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRG
RNKPPEGLVFMPFFDESQLVNKLTLDATCPISKETDYKKCAVKVSKA