Protein Info for AMB_RS12020 in Magnetospirillum magneticum AMB-1

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 895 TIGR01063: DNA gyrase, A subunit" amino acids 12 to 865 (854 residues), 1186.2 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 36 to 500 (465 residues), 523.1 bits, see alignment E=6e-161 PF03989: DNA_gyraseA_C" amino acids 532 to 579 (48 residues), 56.8 bits, see alignment 1.2e-19 amino acids 584 to 631 (48 residues), 20.9 bits, see alignment 2e-08 amino acids 642 to 686 (45 residues), 28.2 bits, see alignment 1.1e-10 amino acids 690 to 736 (47 residues), 47 bits, see alignment 1.4e-16 amino acids 769 to 816 (48 residues), 34.9 bits, see alignment 8.2e-13 amino acids 819 to 864 (46 residues), 48.8 bits, see alignment 3.7e-17

Best Hits

Swiss-Prot: 62% identical to GYRA_RICFE: DNA gyrase subunit A (gyrA) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 72% identity to azl:AZL_013920)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W4P0 at UniProt or InterPro

Protein Sequence (895 amino acids)

>AMB_RS12020 DNA gyrase subunit A (Magnetospirillum magneticum AMB-1)
MTTLPASPQFDITPVTIEDEMKRSYLDYAMSVIVSRALPDVRDGLKPVHRRILFAMNEAG
YHFNKPFRKSARIVGDVMGKYHPHGDSAIYDAMVRMAQNFSMRLPLVDGQGNFGSMDGDP
PAAMRYTEARLARSAHSLLEDIDKETVDFQANYDESTHEPMVLPARYPNLLVNGAGGIAV
GMATNIPPHNLGEVIDACCAYIDDPEVTVERLMELVPGPDFPTGGTILGRAGIRAAQLTG
RGSVVMRGRVHIEEVRKDREAIVVTEIPYQVNKARMLEQIAELVRDKKIEGISDLRDESD
RDGVRMVVEIKRDAIAEVVLAQLYRFTPLQTSFGVNSLALNGGRPEMMTLRDIIVAFIAF
REEVITRRTIFELGKARDRAHVLAGLAIAVANIDEVIRLIRAAPDPGEAREKLMTRAWPV
GDVEPLIRLIDEPGRAIEDGCYRLSEVQAKAILDLRLHRLTGLERDKIGDELREIGHQIE
AYLLILSSRPRLMEVMRGELMEIREQFATPRRTTIEENEFEADIEDLIAREDMVVTVTNT
GYIKRVPLSAYRAQKRGGKGRSGMNIKDEDFLTQVFVVNTHTPVLFFSSTGIAYKLKVYR
LPLGNPQARGKAMVNLLPLDDGETISTIMPLPEDETTWGDLHVMFVTSKGDVRRNPLSDF
TEVRANGKIAMKLGEGERLISVQTCSAESDDVLLATHRGKAIRFPVQDVREFKSRDSTGV
RGIRLDGEDEVISMSILRHTDYDSETRDAFLKGDLPADQMDKMVAEQQFILTVTENGFGK
RTSAFEYRITGRGGSGIANLDMTAKTGLVVASFPVNHEDDIMLVSDGGKLIRMPVDDIRI
AGRKTQGVTLLRTAENERVVSAARLCDIGSNGDEPDETDEGAGDAAEDMAGGEDA