Protein Info for AMB_RS11255 in Magnetospirillum magneticum AMB-1

Annotation: hydrogenase iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 transmembrane" amino acids 32 to 54 (23 residues), see Phobius details amino acids 84 to 102 (19 residues), see Phobius details amino acids 114 to 138 (25 residues), see Phobius details amino acids 178 to 202 (25 residues), see Phobius details amino acids 214 to 232 (19 residues), see Phobius details amino acids 266 to 286 (21 residues), see Phobius details PF00033: Cytochrome_B" amino acids 27 to 205 (179 residues), 67.5 bits, see alignment E=6.6e-22 PF13631: Cytochrom_B_N_2" amino acids 91 to 213 (123 residues), 40.5 bits, see alignment E=1.3e-13 PF00037: Fer4" amino acids 296 to 317 (22 residues), 23.9 bits, see alignment (E = 1.2e-08) PF12838: Fer4_7" amino acids 302 to 352 (51 residues), 34 bits, see alignment 1.5e-11 PF13187: Fer4_9" amino acids 302 to 352 (51 residues), 27.7 bits, see alignment 1e-09 PF02662: FlpD" amino acids 412 to 509 (98 residues), 43.4 bits, see alignment E=1.5e-14

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb2225)

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W546 at UniProt or InterPro

Protein Sequence (524 amino acids)

>AMB_RS11255 hydrogenase iron-sulfur subunit (Magnetospirillum magneticum AMB-1)
MSNPIKSAVRAGLFRLESGWDMLVGRESNPMYCLGAMSWFFFWIVGASGLYLFVPYDTSA
VRAWNSIEYISREQWYWGGLIRSLHRYGSDAMVLTMMLHLVREWCLDRYHGARWFAWFTG
VPLIWMVFSSGITGYWLVWDELAQYLAIGTAEWMDFLGIFGQSIARNFLNPGALTDRFFT
LLIFIHIAVPLFLLMAMWVHILRINRANTNPPRPLVIGSSIMLVALSLLHPAQSHPAADL
GKAIGDLNPDWYFMALYPLYDAKGPLVTWAVSIGASLFLSLMPWMAFRRKRRAAAVVTSD
LCNGCGNCASDCPFGAVVLRPRTDGLHSSPKLAVMQPDLCTSCGMCMASCNKTNPFLPHT
GETRQTAIDIPDFTFDLMVKRVSARTHGLVGKNRVLIIGCEHGAVLDHLKGPSVGVLPLH
CTGMMPPSLIDYVFNKDLADGVLVTACRPGECFYRLGPEWTELRMNGERVPKLRGAVPRD
RVRLFWAASTETKALMAELAAFRVALEELAPMERPESTKRRVAE