Protein Info for AMB_RS08280 in Magnetospirillum magneticum AMB-1

Annotation: hydrogenase expression/formation protein HypE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 TIGR02124: hydrogenase expression/formation protein HypE" amino acids 18 to 349 (332 residues), 462.9 bits, see alignment E=2.7e-143 PF00586: AIRS" amino acids 51 to 163 (113 residues), 84 bits, see alignment E=1e-27 PF02769: AIRS_C" amino acids 175 to 327 (153 residues), 94.9 bits, see alignment E=5.9e-31

Best Hits

Swiss-Prot: 72% identical to HYPE_CUPNH: Carbamoyl dehydratase HypE (hypE) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K04655, hydrogenase expression/formation protein HypE (inferred from 100% identity to mag:amb1636)

MetaCyc: 48% identical to carbamoyl dehydratase HypE (Escherichia coli K-12 substr. MG1655)
4.2.1.M8 [EC: 4.2.1.M8]; RXN-22648 [EC: 4.2.1.M8]

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypE" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.M8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W6T5 at UniProt or InterPro

Protein Sequence (349 amino acids)

>AMB_RS08280 hydrogenase expression/formation protein HypE (Magnetospirillum magneticum AMB-1)
MTLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAG
GRMVMTTDGYVISPLFFPGGDIGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLK
RIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGIGMVPPGVEISGDKARSGDAI
LVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAA
TLNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLD
IMRAHPLGADAARIGACREDSHHFVQMKTRMGGNRVVDWLAGEQLPRIC