Protein Info for AMB_RS07985 in Magnetospirillum magneticum AMB-1

Annotation: nitrogenase cofactor biosynthesis protein NifB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 46 to 484 (439 residues), 653.7 bits, see alignment E=6.5e-201 PF04055: Radical_SAM" amino acids 72 to 250 (179 residues), 67.4 bits, see alignment E=1.8e-22 PF02579: Nitro_FeMo-Co" amino acids 389 to 483 (95 residues), 76 bits, see alignment E=2.3e-25

Best Hits

Swiss-Prot: 68% identical to NIFB_RHOCA: FeMo cofactor biosynthesis protein NifB (nifB) from Rhodobacter capsulatus

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 100% identity to mag:amb1583)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W6Y8 at UniProt or InterPro

Protein Sequence (498 amino acids)

>AMB_RS07985 nitrogenase cofactor biosynthesis protein NifB (Magnetospirillum magneticum AMB-1)
MSSNVISLSSIARGPMVPAATPAAPTGGCSSSSCGSDKGQGDMPAHIWDKVKDHPCYSEE
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGVTSEKLTPEQAARKVAAVAAKVPQ
LSVLGIAGPGDSMFDWKKTAATFREVSKLLPDLKYCVSTNGLALPDHLDELAGHNIDHVT
ITINMVDPEIGALIYPWIYFKGKRYTGIEGARILHERQMEGLEGLTSRGILTKINSVMIP
GINDQHLIEVNKEIKARGAFLHNVMPLISDAAHGTAFGLSGQRGPKPAELKDLQDKLAGG
ANLMRHCRQCRADAVGMLGEDLSQDFTLDKLPEVATAYDPEPRRLYREVVERERADRAKA
LEGAEAELAEAVAEIEAPAKLVAVCTKGGGRINQHFGHAKEFQIFEVDAKGVRFSGHRKA
DNYCQGGYGDDDKLDVVLAALEGIETVFVAKIGRCPKDDLKKAGIEAVDAYPFEYIETAV
AGWYAQRFGQVSDTADIA