Protein Info for AMB_RS07910 in Magnetospirillum magneticum AMB-1

Annotation: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 TIGR01283: nitrogenase MoFe cofactor biosynthesis protein NifE" amino acids 3 to 457 (455 residues), 616.5 bits, see alignment E=1.3e-189 PF00148: Oxidored_nitro" amino acids 43 to 440 (398 residues), 334 bits, see alignment E=6.4e-104

Best Hits

Swiss-Prot: 64% identical to NIFE_RHILO: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (nifE) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K02587, nitrogenase molybdenum-cofactor synthesis protein NifE (inferred from 100% identity to mag:amb1569)

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifE" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W702 at UniProt or InterPro

Protein Sequence (460 amino acids)

>AMB_RS07910 nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (Magnetospirillum magneticum AMB-1)
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVH
LVHGPIACEGNSWDGRNSWSSGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNP
PAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPGFVGSKNLGNRLGGEALFEHV
IGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRT
EALIAEEEAKAWARLEPYKARLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATE
ADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLSGGRSQFVALKARTPWIDINQ
ERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS