Protein Info for AMB_RS06505 in Magnetospirillum magneticum AMB-1

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 7 to 131 (125 residues), 37.1 bits, E=7.7e-13
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 437 (431 residues), 487.4 bits, E=2.2e-150
• PF12804: NTP_transf_3
  • amino acids 8 to 141 (134 residues), 73.2 bits, E=7.1e-24
• PF00483: NTP_transferase
  • amino acids 8 to 153 (146 residues), 21.4 bits, E=4.3e-08
• PF00132: Hexapep
  • amino acids 266 to 298 (33 residues), 34 bits, see alignment (E = 3.9e-12)
  • amino acids 380 to 413 (34 residues), 34.5 bits, 2.8e-12
• PF14602: Hexapep_2
  • amino acids 381 to 413 (33 residues), 35 bits, see alignment (E = 2.4e-12)

Best Hits

Swiss-Prot: 100% identical to GLMU_MAGSA: Bifunctional protein GlmU (glmU) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to mag:amb1280)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.157, 2.7.7.23

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See Q2W7U1 at UniProt or InterPro

Protein Sequence (449 amino acids)

>AMB_RS06505 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Magnetospirillum magneticum AMB-1)
MASSKLAVIVLAAGMGTRMKSSLPKVMHPLAGRPMVSHLLDTVSGLSPDRMVVVVGPDME
LVGKTVAPFPTVIQADRLGTGHAVLQAKAALGQFDGDVLVLYGDTPLITRATLERMLAER
RGPRDPAVVVLGFKPQDPGHYGRLVVGAEGLKAIVEYRDATPDQRENPLCNSGVMAIDGN
RLWSLIERVDNKNSKGEYYLTDIVALARADGATCTHVEGDEAELLGVNARSELAVAEALV
QARLREAAMDNGATLIDPATVWFSWDTKIGRDVTIWPHVVFGPGVTVGDNVEIKGFCHFE
GCMVEAGVAAGPFTRLRPGAEIGEGAHIGNFVEVKKATVEAGAKINHLAYVGDARVGAGA
NVGAGTITCNYDGFNKSFTDIGAGAFIGSNTSLVAPVKVGDGAVVGAGSVITKEVTPGAL
AVARGSQMELKGWAERFRAQQAAKKAKKD