Protein Info for AMB_RS05705 in Magnetospirillum magneticum AMB-1

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 764 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 738 (736 residues), 1140.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 42.6 bits, see alignment 3.8e-14 PF00004: AAA" amino acids 215 to 347 (133 residues), 46.1 bits, see alignment E=4.9e-15 amino acids 496 to 614 (119 residues), 46 bits, see alignment E=5e-15 PF17871: AAA_lid_9" amino acids 355 to 456 (102 residues), 95.1 bits, see alignment E=1.6e-30 PF07724: AAA_2" amino acids 490 to 650 (161 residues), 196.6 bits, see alignment E=2.2e-61 PF00158: Sigma54_activat" amino acids 496 to 613 (118 residues), 21.7 bits, see alignment E=9.8e-08 PF07728: AAA_5" amino acids 496 to 613 (118 residues), 51.4 bits, see alignment E=8.4e-17 PF10431: ClpB_D2-small" amino acids 657 to 737 (81 residues), 92.7 bits, see alignment E=8.5e-30

Best Hits

Swiss-Prot: 71% identical to CLPA_RHOBL: ClpA homolog protein from Rhodobacter blasticus

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 79% identity to azl:AZL_006860)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W898 at UniProt or InterPro

Protein Sequence (764 amino acids)

>AMB_RS05705 ATP-dependent Clp protease ATP-binding subunit ClpA (Magnetospirillum magneticum AMB-1)
MLSRNLEQSLHRALSHATERRHEYATLEHLLLALTDDQDAVAVLRACNVDVDKLKRDLGE
FIDTNLSELVSPRGTDPKPTAGFQRVVQRAAIHVQSSGREEVTGANVIVALFSERESHAV
YFLQSQDMTRLDAVNYISHGVAKAPGRGQNRTVHGADDEANPDKVVKKGQEALNAYCINL
NKKAAQGKIDPLIGRDEEIDRTIQILCRRSKNNPLYVGDPGVGKTAIAEGLARRIVNGEV
PDVLKNATIFALDMGSLLAGTRYRGDFEERLKAVVTELENYDGAVMFIDEIHTVIGAGAT
SGGSMDASNLLKPALASGSLRCIGSTTYKEFRNHFEKDRALVRRFQKIDVNEPSIPDTIK
ILNGIKTYYESHHKVRYTAEAIKAAVELSAKYITDRKLPDKAIDVIDEVGASRMLLPESK
RRKTVTVRDVEEIVAKIARIPPKSVSTNDVEALRNLERDLKTLVFGQDKAIEALASAIKL
ARAGLREPEKPIGCYLFSGPTGVGKTEVARQLAKIMGIELTRFDMSEYMERHSVSRLIGA
PPGYVGFDQGGLLTDAIDQHPHSVLLLDEIEKAHPDLFNILLQVMDHGRLTDHNGKTVDF
RNVILIMTTNAGAADLAKSAIGFGRETREGDDTDAINRMFSPEFRNRLDSTIAFANLTPE
IVAQVVDKFVMQLETQLGDRDVTIELTDEARAWLAKKGYDRSFGARPLARVIQEHIKKAL
AEELLFGRLSKGGIVRVRVGEDGKLAFEFLASASKTDKIPELVE