Protein Info for AMB_RS03695 in Magnetospirillum magneticum AMB-1

Annotation: NADP-dependent malic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 19 to 111 (93 residues), 66.3 bits, E=4.8e-22
  • amino acids 115 to 151 (37 residues), 24.4 bits, 3.5e-09
• PF03949: Malic_M
  • amino acids 165 to 359 (195 residues), 81.9 bits, E=7.5e-27
• PF01515: PTA_PTB
  • amino acids 429 to 749 (321 residues), 305.4 bits, E=8.2e-95

Best Hits

Swiss-Prot: 63% identical to MAO2_ECOLI: NADP-dependent malic enzyme (maeB) from Escherichia coli (strain K12)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 100% identity to mag:amb0720)

MetaCyc: 63% identical to malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (Escherichia coli K-12 substr. MG1655)
RXN-16819 [EC: 4.1.1.101]; Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)). [EC: 4.1.1.101, 1.1.1.40]

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• protocatechuate degradation I (meta-cleavage pathway) (8/8 steps found)
• methylgallate degradation (6/6 steps found)
• superpathway of vanillin and vanillate degradation (8/10 steps found)
• gluconeogenesis I (10/13 steps found)
• L-malate degradation I (1/1 steps found)
• syringate degradation (8/12 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.40

Use Curated BLAST to search for 1.1.1.40 or 4.1.1.101

Sequence Analysis Tools

See Q2W9F1 at UniProt or InterPro

Protein Sequence (760 amino acids)

>AMB_RS03695 NADP-dependent malic enzyme (Magnetospirillum magneticum AMB-1)
MSDELRDAALEYHRLPTPGKISVTPTKPLATQRDLALAYSPGVAAACELIVADEDSAADV
TARGNLVAVVTNGTAVLGLGPIGPLAAKPVMEGKGVLFKKFAGIDVFDMELAELDPDKLV
NIIAAMEPTFGAINLEDIKAPECFEVERKLQERVKIPVMHDDQHGTAIVVGAAMVNALRV
VGKDIADVKLVASGAGAAALACLNLLVKLGVKRENVWVTDIKGVVYEGRTELMDEYKSIY
AKKTDMRTLAEVIEGADIFLGLSAPRVLTGEMVDKMAQKPIVFALANPTPEILPDEVKAV
RPDAIIATGRSDYPNQVNNVLVFPYIFRGALDVGATEINDAMKLACVYALANLAMAESDE
RVRAAYGTAPLTFGPEYLIPKPFDSRLIIKIAPAVAKAAMESGVARRPIIDFDVYMDRLN
QFVFRSGLVMKPVFDRARQDMRRVVYCEGEGRRVLHAAQTVVDEGLARPVLIGRREVVEK
RIQDLDLRLRIDQDFDLCDPEDDPRFNEYWRLYHSIMERQGVSPEYARTVVRTRNTVIGT
LMLKRKEVDAMICGTIGRYDKHLSHILNVIGTREGVKVPAAMNLLIMPIGTFFICDTYVT
PEPTPEQICDMTLLASEEVRRFGIEPKVAFLSHANFGNRQTASAQRQRDALALLREKAPY
LEAEGEMHGDAALSEEIRTRIFPNSKLKGAANLLVMPTLDAANISFNLLKVAGDALSVGP
ILMGIAQPAHVLTPSATVRNIINITALAAVDAQMHAGRGR