Protein Info for AMB_RS03050 in Magnetospirillum magneticum AMB-1

Annotation: ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR01251: ribose-phosphate diphosphokinase" amino acids 1 to 310 (310 residues), 388.4 bits, see alignment E=9.2e-121 PF13793: Pribosyltran_N" amino acids 1 to 117 (117 residues), 173.4 bits, see alignment E=2.1e-55 PF00156: Pribosyltran" amino acids 152 to 250 (99 residues), 66.8 bits, see alignment E=2.2e-22 PF14572: Pribosyl_synth" amino acids 200 to 309 (110 residues), 92.9 bits, see alignment E=3.6e-30

Best Hits

Swiss-Prot: 76% identical to KPRS_GRABC: Ribose-phosphate pyrophosphokinase (prs) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 79% identity to rru:Rru_A0645)

MetaCyc: 54% identical to ribose-phosphate diphosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose-phosphate diphosphokinase. [EC: 2.7.6.1]

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>AMB_RS03050 ribose-phosphate pyrophosphokinase (Magnetospirillum magneticum AMB-1)
MKILACNSNRPLAEAIAEYLTMSITKAVIRRFSDNEVFVEIHENVRGEDVFVIQSTSYPA
NDNLMELLVTLDALRRGSARRITAVIPYFGYARQDRKSSPRSPISAKLVANLITTAGADR
VVTLDLHSGQIQGFFDIPLDNLYAAPVFTNDIRARYQGDDVMVVSPDVGGVVRARAIASR
IDADLAIIDKRREKAGVSEVMNIIGDVRGRRCIMVDDIVDSAGTLCNAAEALMKAGAVSV
AAFVTHGVLSGGAVARVTASPLEELVITDSIPATEAVKMARNIRQVTIAPLMAESISRIS
EERSVSSLFD