Protein Info for AMB_RS02745 in Magnetospirillum magneticum AMB-1

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 819 PF07992: Pyr_redox_2" amino acids 8 to 288 (281 residues), 195.1 bits, see alignment E=7.6e-61 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 9 to 801 (793 residues), 920.1 bits, see alignment E=6.8e-281 PF00070: Pyr_redox" amino acids 150 to 232 (83 residues), 53.9 bits, see alignment E=8.8e-18 PF01134: GIDA" amino acids 152 to 182 (31 residues), 20 bits, see alignment (E = 1.3e-07) PF18267: Rubredoxin_C" amino acids 322 to 390 (69 residues), 67 bits, see alignment E=4.7e-22 PF04324: Fer2_BFD" amino acids 429 to 477 (49 residues), 53.7 bits, see alignment 8.8e-18 amino acids 491 to 540 (50 residues), 41.8 bits, see alignment 4.6e-14 PF03460: NIR_SIR_ferr" amino acids 566 to 629 (64 residues), 51.4 bits, see alignment 2.9e-17 PF01077: NIR_SIR" amino acids 639 to 777 (139 residues), 116.2 bits, see alignment E=4e-37

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to mag:amb0533)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9Y8 at UniProt or InterPro

Protein Sequence (819 amino acids)

>AMB_RS02745 nitrite reductase large subunit (Magnetospirillum magneticum AMB-1)
MNALPRQKLVVIGNGMAGMRTVEELLAIAPARYDITVFGAEPHPNYNRIMLSSVLAGEKQ
VDDIVINPRAWYAENGITLHTGDPVVAIDRAAKTVTSANGVVVPYDKLLLSTGSKPLMPP
LPGLDLPGVVAFRDIADVEKMLDAAEAKQRAVVIGGGLLGLEAAWGLKRRGMPVALVHLM
PTLMERQLDVEAGGLLQKDLTERGLHFFTSGQTEEIMGAETGRAQGVKLTDGREIPADLV
VVAIGIRPNVDLAKAAGLDINRGIEVGDDMATSDPAIYSVGECVEHRGQIFGLVAPIWEQ
AKVCASRLAGRDDAHYETPPLSTRLKITGIDVFSAGQLAAQDEADEELVYRDSARGIYKK
LVIRADKVVGAVMYGDVADGSWYFQLIREKADVAAIRDRMIFGQAYADTTCKGHAGGVNV
AATSDDAQVCGCNGVSKGAIVKAITEKGLTSLDEVKLHTKASASCGQCASVVQAILTHVT
GEVVDAKAAGMCGCTDLSHDEVRREILHQGLKTQDAVRQALGWRHADGCAKCRPALNYYL
LCAWPSEYVDDYQSRFINERAHANIQKDGTYSVIPRMWGGLTNPTELRAIADVVDKFKIP
TVKVTGGQRLDLLGVRKEDLPGVWAVLGKAGLVSGHGYAKGLRTVKTCVGSEWCRFGTQD
STGLGVKLEKLMWGSWTPHKVKLAVSGCPRNCAEATIKDMGVVCVEAGYDISVGGNGGIE
LRGTDHLVRVTSEEEVLEYCGAFMQIYREEARYLERTAPWVERVGMDYITKRVVEDAEGR
RAAFARFVFSQNYAQIDPWAERASGEVDAHEFKTLAEVG