Protein Info for AMB_RS02530 in Magnetospirillum magneticum AMB-1

Annotation: U32 family peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 PF01136: Peptidase_U32" amino acids 27 to 318 (292 residues), 224.4 bits, see alignment E=9.1e-71

Best Hits

Swiss-Prot: 50% identical to YHBU_ECOLI: Uncharacterized protease YhbU (yhbU) from Escherichia coli (strain K12)

KEGG orthology group: K08303, putative protease [EC: 3.4.-.-] (inferred from 100% identity to mag:amb0491)

Predicted SEED Role

"Collagenase and related proteases"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WA30 at UniProt or InterPro

Protein Sequence (343 amino acids)

>AMB_RS02530 U32 family peptidase (Magnetospirillum magneticum AMB-1)
MASLPSPSPSTSTSSTRPELVCPAGTPAALRSAVDAGADCVYVGFRDETNARNFPGLNFN
RKELGEGIAYAHERGAKVLVAINTFPRAGQPEIWHQAVADAAKLKADAAILADIGLIEHA
HRTHPELRLHLSVQAAASNPDAIRFYAERFGVRRVVLPRVLTVQEIAALNPRIAPVETEV
FVFGGLCVMAEGRCALSSYVTGQSPNMNGVCSPAGEVRYTETSQGITSELGGFTINKFAH
DEAAGYPTLCKGRFIAHDKASYLFEEPTSLNTLTMLPDLIKAGVTAFKIEGRQRGRAYVA
AVVQAFRAGVDAVMAGKPLPDIDLDRITEGGKQTTGAYEKAWR