Protein Info for AMB_RS01375 in Magnetospirillum magneticum AMB-1

Annotation: peptide chain release factor 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 TIGR00503: peptide chain release factor 3" amino acids 3 to 526 (524 residues), 705.5 bits, see alignment E=3.6e-216 PF00009: GTP_EFTU" amino acids 12 to 275 (264 residues), 168 bits, see alignment E=4.6e-53 PF01926: MMR_HSR1" amino acids 14 to 141 (128 residues), 24.3 bits, see alignment E=7e-09 TIGR00231: small GTP-binding protein domain" amino acids 15 to 158 (144 residues), 79.4 bits, see alignment E=2.6e-26 PF22042: EF-G_D2" amino acids 293 to 379 (87 residues), 85.9 bits, see alignment E=4e-28 PF03144: GTP_EFTU_D2" amino acids 312 to 377 (66 residues), 35.5 bits, see alignment E=2.9e-12 PF16658: RF3_C" amino acids 385 to 512 (128 residues), 151.5 bits, see alignment E=2.9e-48

Best Hits

Swiss-Prot: 56% identical to RF3_DECAR: Peptide chain release factor 3 (prfC) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K02837, peptide chain release factor 3 (inferred from 100% identity to mag:amb0271)

Predicted SEED Role

"Peptide chain release factor 3"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WAQ0 at UniProt or InterPro

Protein Sequence (528 amino acids)

>AMB_RS01375 peptide chain release factor 3 (Magnetospirillum magneticum AMB-1)
MSTLSDAIALRRTFAIISHPDAGKTTLTEKLLMFGGAIQMAGAVRARGEQRRTRSDWMAI
EKERGISVSASVMSFEYSGLSFNLLDTPGHEDFSEDTYRTLTAVDSAVMVLDAAKGIETQ
TLKLFEVCRLRDVPIITFVNKVDREGRETFDLLHEVEKTLALDVCPVTWPIGMGRDLKGV
YDLVNDRVIMFDPGRGDHIAEISVDASLDSPKLDEVLGKAAADRLREEVELVRGGYPEFD
LESFRAGHLTPVFFGSALKTFGVAELLKGIGQLAPPPVARPTDKRVVEPDEAKVSGFVFK
VQANMDPNHRDRIAFFRICSGRFKRGMKVKHVRSGKVMALVNPVFFLARDRELAEEAFPG
DIMGIPNHGQLRIGDTLSESEDMNFLGIPTFAPEVLRRVRLDDPMKAKQLKKALEDLAEE
GVSQVFKPLDGSTWIVGVVGPLQFDVLTTRIESEYNIKAGFEDGGFVTARWVAADTEAEM
KRFMDGNKSNMAEDRDGGMVYLARNSWQLNRAQQDFEKIRFTATREQH