Protein Info for AMB_RS01115 in Magnetospirillum magneticum AMB-1

Annotation: beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 PF00232: Glyco_hydro_1" amino acids 15 to 449 (435 residues), 490.4 bits, see alignment E=2.1e-151 TIGR03356: beta-galactosidase" amino acids 17 to 443 (427 residues), 599.3 bits, see alignment E=2.1e-184

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to mag:amb0219)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21, 3.2.1.23

Use Curated BLAST to search for 3.2.1.21 or 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WAV2 at UniProt or InterPro

Protein Sequence (453 amino acids)

>AMB_RS01115 beta-glucosidase (Magnetospirillum magneticum AMB-1)
MTVAKAAQSAPQGARQFPKDFFWGASTAAYQIEGAYDTDGRGMTIWDKFTADGKIMDGSS
AKVACDHYHRYPEDIALMKAAGFNAYRFSLAWPRIIPAGTGAINAKGLDFYDRLVDKILE
AGIKPMACLYHWDLPQPLEDKGGWQGRDIVGPFAEYARIATKRLGDRVKDWYMLNEPNVV
AIIGYGIGEHAPGYKLGEDGILKALHHQNLAQGTALRAIRAEHSDAVLGTVINLQPCRSQ
DDDPKNRAAAIRWDAVWNRVPLDGVMRGAIPDVLAEKMAHIVKPGDMETIKFPIDMLGIN
YYSRMTMKHEEGHPFDVFWGDAHCDRWTAMAWPVQPDGLYDLLREFKELYGNPAVFIAEN
GAAYDDVVTPDGQVHDAERVAFIRDHVSEVARAVKDGCNVKGYLVWSLLDNFEWAYGLSK
RFGIVRVDYETLKRTPKDSYKWFAEVIRTGRVG