Protein Info for AMB_RS00670 in Magnetospirillum magneticum AMB-1

Annotation: 3-deoxy-manno-octulosonate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 PF02348: CTP_transf_3" amino acids 5 to 217 (213 residues), 135.4 bits, see alignment E=2.6e-43 PF12804: NTP_transf_3" amino acids 19 to 122 (104 residues), 37.9 bits, see alignment E=2.1e-13

Best Hits

Swiss-Prot: 36% identical to KDSB_FRATF: 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) from Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)

KEGG orthology group: K00979, 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) [EC: 2.7.7.38] (inferred from 100% identity to mag:amb0133)

Predicted SEED Role

"3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38)" in subsystem KDO2-Lipid A biosynthesis (EC 2.7.7.38)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.38

Use Curated BLAST to search for 2.7.7.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WB38 at UniProt or InterPro

Protein Sequence (255 amino acids)

>AMB_RS00670 3-deoxy-manno-octulosonate cytidylyltransferase (Magnetospirillum magneticum AMB-1)
MTRIIGLIPARMAASRFPGKPLFPILGRPMIEHVFERAKLFGRWDALAICTCDEEIRAFA
QSKGYPVIMTSDKHTRALDRVAEAATKCGVDVADSDIVLNVQGDEPMMHPDMIAATIKPM
EERSEVRGTMLAMDIVDEAQFRNPDALKIIHDLSGRVLYTSRQPIPHCKTFGPELEAKRI
YGIFGFKWDFLKLFTELPPSPLEIKEACDSNRLYDYGHHQHIAPYPFRPSFSVDSPHDIG
IVEAAMKDDPLWGTY