Protein Info for ABZR88_RS18730 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 5 to 345 (341 residues), 303.8 bits, E=1.1e-94
• TIGR00464: glutamate--tRNA ligase
  • amino acids 5 to 498 (494 residues), 482.2 bits, E=9.2e-149
• PF19269: Anticodon_2
  • amino acids 367 to 500 (134 residues), 109.2 bits, E=2.4e-35

Best Hits

Swiss-Prot: 50% identical to SYE_PARD8: Glutamate--tRNA ligase (gltX) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 70% identity to psn:Pedsa_2827)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (12/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (12/36 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Protein Sequence (506 amino acids)

>ABZR88_RS18730 glutamate--tRNA ligase (Mucilaginibacter yixingensis YX-36 DSM 26809)
MTATKVRVRFAPSPTGGLHLGGVRTVLFNYLFARKNGGDFVLRIEDTDQTRYVEGAEQYI
MDCLKWCGLNPDESPLVGGPFAPYRQSERKALYREYAERLVMQGHAYYAFDTPEELEQNR
KEIANFQYGQAYRETLRNSLTLSEHEVDQLLDAGTPHVIRIKMPTDEHVGFDDLIRGHVS
FDTNLVDDKVLLKADGMPTYHLAVVVDDYLMQITHAFRGDEWLPSAPVHLLLWKYLGWYE
HMPKWAHLPLILKPDGHGKLSKRDGARLGFPVFAMNWFDAKSGELTPGFRELGFLPEAFV
NLLAMLGWNDGTDQEIFSLDELIEKFSLERVSKAGAKFDFEKAKWYNAEWIKKQDAGKLK
PLVAATLTEKGIEITNDDYLLQVINLVKDRCTLLPDFYQQSAYFFAAPAEYDLNAVKPKW
TDAKTAFFKELSDKIQALPVLEAHPFEEQFKAFTEEKGFKTGDVMLPFRIMLVGGKFGPH
VFDIAATLGKDETIKRIALALEAFTA