Protein Info for ABIE41_RS19270 in Bosea sp. OAE506

Annotation: O-acetylhomoserine aminocarboxypropyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 8 to 425 (418 residues), 614.7 bits, E=3.5e-189
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 424 (415 residues), 441.6 bits, E=4.7e-136
• PF00155: Aminotran_1_2
  • amino acids 59 to 224 (166 residues), 34.6 bits, E=3.2e-12
• PF01212: Beta_elim_lyase
  • amino acids 61 to 223 (163 residues), 33.8 bits, E=5.9e-12
• PF01041: DegT_DnrJ_EryC1
  • amino acids 63 to 188 (126 residues), 37.7 bits, E=3.7e-13
• PF00266: Aminotran_5
  • amino acids 65 to 215 (151 residues), 27.3 bits, E=4.5e-10

Best Hits

Swiss-Prot: 54% identical to METY_LEPME: O-acetyl-L-homoserine sulfhydrylase (metY) from Leptospira meyeri

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 84% identity to mrd:Mrad2831_5757)

MetaCyc: 54% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (14/18 steps found)
• aspartate superpathway (19/25 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
• L-methionine biosynthesis I (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (425 amino acids)

>ABIE41_RS19270 O-acetylhomoserine aminocarboxypropyltransferase (Bosea sp. OAE506)
MTDRAPGFHTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRI
GNPTNAVLEERVAALEGGTAALAVASGHAAEFLCFHALMQPGDEFVAAKKLYGGSINQFN
HSYKNFGWNVIWADSDDLEAFAAAVTPKTKAIFIESIANPGGVVVDIAGISAIAKKHNIP
LIVDNTMATPYLVRPFEHGADIVVHSLTKFLGGHGNSIGGIIVDGGSFNWVGDERYPMLS
KPRPEYNGMVLAETFGNFAFAIATRVLGLRDMGPALSPFNAFMILTGIETLPLRMQRHCE
SALAVATHLSKHPAVEWVSYPGLPGDKYHELAKRYSPKGAGAVFTFGLKGGYDAGVKLVT
ELKLFSHLANIGDTRSLVIHPASTTHRQLTDEQKTASGAGPQVIRLSIGLEDVADLIDDL
DQALA