Protein Info for ABIE41_RS19160 in Bosea sp. OAE506

Annotation: class II fructose-bisphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 TIGR00330: fructose-1,6-bisphosphatase, class II" amino acids 14 to 323 (310 residues), 331.9 bits, see alignment E=2.1e-103 PF03320: FBPase_glpX" amino acids 16 to 323 (308 residues), 444.6 bits, see alignment E=7.8e-138

Best Hits

Swiss-Prot: 51% identical to GLPX_BACSU: Fructose-1,6-bisphosphatase class 2 (glpX) from Bacillus subtilis (strain 168)

KEGG orthology group: K02446, fructose-1,6-bisphosphatase II [EC: 3.1.3.11] (inferred from 74% identity to mno:Mnod_3263)

MetaCyc: 43% identical to fructose bisphosphatase F-I monomer (Synechocystis sp. PCC 6803)
Sedoheptulose-bisphosphatase. [EC: 3.1.3.37]; Fructose-bisphosphatase. [EC: 3.1.3.37, 3.1.3.11]

Predicted SEED Role

"Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 3.1.3.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.11 or 3.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (332 amino acids)

>ABIE41_RS19160 class II fructose-bisphosphatase (Bosea sp. OAE506)
MSDDLRISSSQIIERYLSMELVRVTERAAVSAARLRGHGNEKAADQAAVDAMRRELNRLP
IDGEIVIGEGERDEAPMLFIGEKVGTRQGPKVHIAVDPLEGTTLCAKDMPDSIAVMAMAE
AGKLLNAPDVYMDKIAIGPGYARGVVDLDASATDNIHALAKAKGVKPSEISTLIMDRPRH
AKLIEEVRKTGCSIRLITDGDVAGVIYCTEPHKTGIDLYLGIGGAPEGVLAAAALRCVGG
QMQGRLILDTEEKRARALTMGITDPNKKYSMEEMASGDTIVCATGVTNGGMLKGVRFGKE
VIETETIVYRSITGTVRRIHGEHRDFGKFKLD