Protein Info for ABIE40_RS27785 in Rhizobium sp. OAE497

Annotation: putative N-acetylmannosamine-6-phosphate 2-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04131: NanE
  • amino acids 31 to 218 (188 residues), 241 bits, E=6.3e-76
• PF00480: ROK
  • amino acids 232 to 505 (274 residues), 168.9 bits, E=1.9e-53

Best Hits

Swiss-Prot: 54% identical to NANEK_BRUME: Bifunctional enzyme NanE/NanK (nanEK) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: None (inferred from 71% identity to mci:Mesci_2621)

Predicted SEED Role

"N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9)" in subsystem Sialic Acid Metabolism (EC 5.1.3.9)

MetaCyc Pathways

• superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation (4/6 steps found)
• N-acetylneuraminate and N-acetylmannosamine degradation I (2/4 steps found)
• superpathway of N-acetylneuraminate degradation (12/22 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.9

Protein Sequence (507 amino acids)

>ABIE40_RS27785 putative N-acetylmannosamine-6-phosphate 2-epimerase (Rhizobium sp. OAE497)
MKREDIRNSLIVSCQPVPDGPMDDARFVIGFANAAAAAGAKAVRIESVAYVKAARPHVKL
PIIGIVKRDLDDSPVRITPFIADVEALADAGADVIAFDATDRTRPAPVEALVTAAKARGK
LTMADCSSLEDARRALAAGVDFVGTTLSGYVGGPEPIEPDIALISAIRALTPYVIAEGRI
RTPEQAAQAADAGALAVVVGSAITRTEHATAWFHQAIDAAFSAREDAGKPVLAIDIGGTK
TMVALVEQDEVLEERVVRTRREEGPDAWLDAIAAETAGWAGHFARAGIAVTGFVEDGNWS
ALNPATLGIPDNYPLVARAEKRLGVPVIAINDAQAAAWGEYRFGSGRGEDIVFLTISTGI
GGGIVIDGRLASGLAGHFGLTRSPSQGVEPLEDMTSGLWIAAQAKAAGHDADAVTVFAEA
EKGAAWAKEIVERSALRVALLCSDIKLMLDPKRIVIGGGIGLAPGYLDQVRSQLGGLSSR
ITPNLVAAELGSRAGIIGAADLARSRA