Protein Info for ABIE40_RS26660 in Rhizobium sp. OAE497

Annotation: thiamine pyrophosphate-requiring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 transmembrane" amino acids 405 to 424 (20 residues), see Phobius details PF02776: TPP_enzyme_N" amino acids 3 to 120 (118 residues), 126.1 bits, see alignment E=9.8e-41 PF00205: TPP_enzyme_M" amino acids 198 to 328 (131 residues), 94.6 bits, see alignment E=6.5e-31 PF02775: TPP_enzyme_C" amino acids 387 to 542 (156 residues), 99.7 bits, see alignment E=2.2e-32

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 77% identity to rec:RHECIAT_PC0000313)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.3.3, 1.2.5.1

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (596 amino acids)

>ABIE40_RS26660 thiamine pyrophosphate-requiring protein (Rhizobium sp. OAE497)
MTMTVGDFIVERLHQWGVRRLFGYPGDGINGVFGALARAKAKMEFIQTRHEEMAAFMASA
HAKFTGDIGVCIATSGPGATHLITGLYDARLDHQPVLAIVGQQSRAAIGGHYQQEVDLAS
LFKDVAGDFVHQAATPAQVRHLIDRAIRIAKANRTVTAVILPNDLQDLPYEEPPRKHGTV
HSGIGFRAARTVPHLEDLQEAADILNQGERVAILVGAGALAATDEVIAVADRLNAGVAKA
LLGKAALPDNLPWVTGSIGLLGTEPSWDLMRDCDTLLMIGSGFPYSEFLPEEGSVKAVQI
DITPSMLSLRYPMDTNLQGDAAQTLKALLPHLRQKEGEWRKSIEAKVAKWWKTLEERAHA
DANPVNPQRVTWELSPRLPDGAIITSDSGSCANWYARDLKIRRGMMASLSGGLASMGAAV
PYALAAKFAHPDRPVIGLVGDGAMQMNNMSELITIAKYWSSWDDPRCVICVFNNEDLNQV
TWEQRVMEGDPKFEASQSIPNVPYHRFAEMIGLKGIYVDRPENVGPAWDEALSSDRPVLL
EVKTDPEVPPLPPHLTFEQMKKITETLAKGDSREAGIVMGTMRQLLSSFWPGSRQS