Protein Info for ABIE40_RS21725 in Rhizobium sp. OAE497

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 26 to 544 (519 residues), 875.3 bits, E=1.2e-267
• PF01642: MM_CoA_mutase
  • amino acids 27 to 540 (514 residues), 798.5 bits, E=2.4e-244
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 579 to 706 (128 residues), 173.9 bits, E=1.5e-55
• PF02310: B12-binding
  • amino acids 582 to 697 (116 residues), 66.7 bits, E=1.8e-22

Best Hits

Swiss-Prot: 84% identical to MUTB_RHIME: Methylmalonyl-CoA mutase (bhbA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 87% identity to rlt:Rleg2_6511)

MetaCyc: 66% identical to methylmalonyl-CoA mutase large subunit (Chloroflexus aurantiacus)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (712 amino acids)

>ABIE40_RS21725 methylmalonyl-CoA mutase (Rhizobium sp. OAE497)
MTKKTVGDWRALAEKELKASPETLVWKTPEGIDVKPLYTPGDLASADHLDSMPGLAPFTR
GPRATMYAGRPWTIRQYAGFSTAEASNAFYRKALAAGQQGVSVAFDLATHRGYDSDHPRV
EGDVGKAGVAIDSVEDMKILFDGIPLEKISVSMTMNGAVIPILASFIVAGEEQGVSRADL
SGTIQNDILKEFMVRNTYIYPPEPSMRIVADIIEYTAREMPKFNSISISGYHMQEAGATL
VQELAFTLADGREYVRAAIAKGLNVDDFAGRLSFFFAIGMNFFMEAAKLRAARLLWTKIM
EEFEPKKASSLMLRTHCQTSGVSLQEQDPYNNIVRTAFEAMSAVLGGTQSLHTNSFDEAI
ALPTEFSARIARNTQLILQHETGVTKVVDPLAGSYYVESLTKELADKAWALIEEVEALGG
MTKAVNEGLPKRLIEEAATRRQAAVDKGDEVIVGVNKYRLETEEELEFLAIDNAAVRAAQ
VKRIEDTRRRRGSGKVRETLEALSEVARSGEGNLLAAAVEAARARATVGEISDAMRQAFG
DHAAVPEVIRDVYGEAYRDQPELEILKARMADISASLGRKPKIMVAKLGQDGHDRGAKVI
ASAFGDVGFDVLAGPLFQTPDEAAGMALSEKVNVIGVSSLAAGHKTLLPQLVDALKARGG
GDVIVVCGGVIPRQDYQFLTDHGVAAVFGPGTNVLDAANSVLDLLQGIRRNT