Protein Info for ABIE40_RS17710 in Rhizobium sp. OAE497

Annotation: D-alanyl-D-alanine carboxypeptidase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00768: Peptidase_S11
  • amino acids 30 to 253 (224 residues), 182.7 bits, E=8.2e-58
• PF13354: Beta-lactamase2
  • amino acids 40 to 185 (146 residues), 39.8 bits, E=3.4e-14

Best Hits

KEGG orthology group: K01286, D-alanyl-D-alanine carboxypeptidase [EC: 3.4.16.4] (inferred from 82% identity to rlg:Rleg_3896)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan maturation (meso-diaminopimelate containing) (3/12 steps found)

KEGG Metabolic Maps

• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Protein Sequence (373 amino acids)

>ABIE40_RS17710 D-alanyl-D-alanine carboxypeptidase family protein (Rhizobium sp. OAE497)
MSTRQNRLFAALRPVSFAVSAAAALLSSVPLAQANPHILVDVQSGRVIEHEEAFRKWYPA
SLTKLMTVYTTFDAIRSGQISLDTPIVMSKQAAAQPAAKMYYKPGQKLTLDSALKILMVK
SANDIAVAVAEAVAGTQDAFVLRMNAEAVKLGMTDSHFVNPNGLPGKGQYTTARDLAILT
VALRRDFPQYAGYFALEGFTNGKTNTPSINLLIGRFAGADGMKTGFICASGFNQIGSATR
NGRTVVSVVLGTDSLAARADATANLLEKAFSSPTSSGDTLRSLKPYGPGQDAVTDISADI
CSAKGAKVRSETRDEVGRMKVQSPYIHEMDHEPNFVFAGLIPGQDPQPDKTTKADAIGDA
ANVPVPLPRPTSF