Protein Info for ABIE40_RS06425 in Rhizobium sp. OAE497

Annotation: leucyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 transmembrane" amino acids 367 to 389 (23 residues), see Phobius details PF02789: Peptidase_M17_N" amino acids 28 to 143 (116 residues), 71.5 bits, see alignment E=6e-24 PF00883: Peptidase_M17" amino acids 182 to 487 (306 residues), 423.4 bits, see alignment E=5.7e-131

Best Hits

Swiss-Prot: 82% identical to AMPA_RHIE6: Probable cytosol aminopeptidase (pepA) from Rhizobium etli (strain CIAT 652)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 82% identity to rec:RHECIAT_CH0001524)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (497 amino acids)

>ABIE40_RS06425 leucyl aminopeptidase (Rhizobium sp. OAE497)
MSVKFDISFAKSAKISGGLTVLLKAGDGELAAGAEIADPAGVIAKASKIAKFSAKAMSAL
DLVAPEGSPADRLFVIGLGKPQDLVAHDWLKLGGIAAGKIKGADKATIFIDAPGAAIDAK
AASGFALGMLMRAYAFDNYKTKKSDEDDKANGKSAKVTIVTADASGAKKALADAEAVAEG
VNLARDLVNEPPNVLGPVEFAAKAKELEKLGVDVEILTEREMKRLGMGALLGVAQGSVRP
PRLAIMHWKGGKAKDRPIAFIGKGVVFDTGGISIKPAAGMEDMKGDMGGAAAVTGLMHVL
AARKAEVNAVGIIGLVENMPDGNAQRPGDIVTSMSGQTIEIINTDAEGRLVLCDALWYCN
DRFKPRFMINLATLTGAIMVALGNVHAGLFSNDDTLSAQLTSAGLAANEKLWRMPLGRDY
DKMIDSKFADMKNTGGRYAGSITAAHFLKRFVQETPWAHLDIAGTALGSPQDEINQSWGS
GFGVRLLDQLVRDHYEA