Protein Info for ABID97_RS29340 in Variovorax sp. OAS795

Annotation: amylo-alpha-1,6-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 transmembrane" amino acids 636 to 647 (12 residues), see Phobius details PF14742: GDE_N_bis" amino acids 27 to 217 (191 residues), 196.2 bits, see alignment E=7.2e-62 PF22422: MGH1-like_GH" amino acids 297 to 604 (308 residues), 81.6 bits, see alignment E=1.3e-26

Best Hits

KEGG orthology group: None (inferred from 88% identity to vap:Vapar_0703)

Predicted SEED Role

"Glycogen debranching enzyme"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (716 amino acids)

>ABID97_RS29340 amylo-alpha-1,6-glucosidase (Variovorax sp. OAS795)
MSMSDEVPATEQAEPAPERAVQHAFVLKEGDTYFVMDGHGDVGGGVDGLFHDDTRMLSTF
VLRLNGARPSLLSSGVSQDNVFFTANLTNHPLPVLGSTSMPQGVIHLERRRFLWGARLYE
RLRLVNYSDQPAEAPLTLQFGADFRDMFEVRGQQRARRGEFGVPDVGVQGVELRYEGLDT
VLRRACINFSQVPQQLDTGAAAFTVPLAAHGHWTLYIEVGNVPALPSAERYRQACALARW
RMRARQRRGSRLESSGRLFQAWLDRSRADLALLTTDLPTGPYPYAGIPWFSTPFGRDAIV
TAFQTLWIDPRLAQGVLEFLAARQSRQTSSFRDAEPGKIMHETRKGEMAAVDELPFGQYY
GGVDTTPLFVALAGAYAQRTADHATIDRIWPALLAACAWMERNMARHPDGLLAYQRGETS
GLVNQGWKDSHDSIFHADGRTPEGPIALVEVQGYVYAAFLAMASIAEWRVEAGAAHWRQR
AAAMRVAVEKRFWLDDLQFYALAIDGGNQPCRVRASNAGHLLFTGLPIMERALAVGRQLL
SPAFASGWGIRTLPVEAVRFNPMSYHNGSVWPHDAALCAAGIARYGERDGVVRILDNLFE
SAAHFGMRLPELFCGFDRQPGQSPIAYPVACLPQAWAAGSAFMLLQACLGLQVDGIRRQV
TIVHPRLPADIDQLHVRQLHVGDARVDLHFQRLEDRIVVVAQTSPGGPKVAVNLQL