Protein Info for ABID97_RS27330 in Variovorax sp. OAS795

Annotation: glycoside hydrolase family 15 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 PF00723: Glyco_hydro_15" amino acids 48 to 86 (39 residues), 25.2 bits, see alignment 3.8e-10 amino acids 165 to 445 (281 residues), 135 bits, see alignment E=1.8e-43

Best Hits

KEGG orthology group: K01178, glucoamylase [EC: 3.2.1.3] (inferred from 88% identity to vap:Vapar_5525)

Predicted SEED Role

"Glucan 1,4-alpha-glucosidase( EC:3.2.1.3 )"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.3

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>ABID97_RS27330 glycoside hydrolase family 15 protein (Variovorax sp. OAS795)
MISGVASAQDPGFEAWLETQLRHSAAAMLTSISPVSIVKHRPGFGQTVRPLPGAIVASPV
LADYNPDPDYFFHWFRDSAVVIDALRLLHAEGRVGDEALQHLRDFIRFSLDLNRLDGRAA
AAVEDRRACVAPELLQYLREDQDLAKAFGDLVAAETRVNPDGTLDACRWNRPQHDGAPLR
ALALLRWVAGGHLDAPLQAQASKLIRFDLGFTLRYWREPSYDIWEEELGHHYYTLRVSGA
ALAEGAAWLEGVGDTALAQRCRDESLAVLRLLDGYWVDEGDGAARGYYRSRALADGRPGP
KALDIAVILSAIHSLGAGDAHGPADPRMQDTLARLDALFDAAYPINHGRSAGRGAAMGRY
DGDVYCSGGAYYFSTLGAAEFCFRAAAAVGGDARQWFERGDAYLATVRAYTPAGGELSEQ
FDQRSGAQTSAKQLAWSHAAFISCVSARRLAAAACQAPQRR