Protein Info for ABID97_RS25190 in Variovorax sp. OAS795

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 PF20423: AceK_regulatory" amino acids 15 to 315 (301 residues), 404.7 bits, see alignment E=2.4e-125 PF06315: AceK_kinase" amino acids 317 to 593 (277 residues), 389.1 bits, see alignment E=9.9e-121

Best Hits

Swiss-Prot: 78% identical to ACEK_ACIAC: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 96% identity to vap:Vapar_4733)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (606 amino acids)

>ABID97_RS25190 bifunctional isocitrate dehydrogenase kinase/phosphatase (Variovorax sp. OAS795)
MFPQRLDSPLAYDIAKAMIDGFNRHYSLFRAESARAKHRFETADWHGQQRAQRERIEFYD
LRVNEAVARLEKEFKAGDQPMEVWHQVKLHFIGLLVDHHQPELAESFFNSVTTKILHRAY
FQNDFIFVRPAISTEYIEPRGAPTYRPYYPTHDTLADTVEQMIDDFALQGGFAHKRRDAE
RVAHAIMGRFHQVKLRANFQIQVLSGLFYRNKGAYVVGKIMNGFVELPFSLPILHDSHGK
FYVDAALFGEDDLQMLFSFARAYFMVDMEVPSACVQFLRSLMPRKPRAEIYNALGLAKQG
KTLFYRDFLSHLNYSSDRFRIAPGIKGMVMLVFDLPSFPFVFKVIKDFYPPQKDTTREQI
KGKYLLVKQHDRVGRMADTLEYSDVGFPLDRFEPELIEEIRKFAPSQLEIADRDGNGEME
LVLKHVYIERRMIPLNIYLQEAFDTLAHPESPAHAKRAREQLEHAVVEYGNAIKDMVAAN
IFPGDMLWKNFGVTRGGKVVFYDYDEIEYITDCRFRKVPAARNEEDEMSGEVWYSVGPKD
VFPETFGPFLLGNDAVREAFMAHHADLLDAAFWQHHKERIEAGQMLDVFPYEEAQRFQHE
GHATHF